Differential Gene Expression Analysis of Strawberry Cultivars that Differ in Fruit-firmness

E.M.J. Salentijn, A. Aharoni, J.G. Schaart, M.J. Boone, F.A. Krens

    Research output: Contribution to journalArticleAcademicpeer-review

    64 Citations (Scopus)

    Abstract

    Firmness is an important selection criterium in the breeding of fruit, including strawberry (Fragaria x ananassa Duch.). Clear differences in fruit-firmness are observed between cultivars. In order to identify candidate genes which might be associated with such textural differences, gene expression levels were compared for a soft and a firm cultivar (cv. Gorella and cv. Holiday, respectively). DNA-microarrays representing 1701 strawberry cDNAs were used for simultaneous hybridization of two RNA populations derived from red ripe fruit of both cultivars. In total 61 clones (3.6 f the total cDNAs on the arrays) displayed differential expression, including 10 clones (8 different ones) which showed homology to cell wall related genes in the public databases. The results from the microarray experiments were further confirmed by RNA gel blots, which were also used to examine gene expression in a third cultivar, Elsanta, showing an intermediate texture phenotype (offspring of a cross between Gorella and Holiday). Interestingly, two genes encoding proteins catalysing successive reactions in lignin metabolism (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase) showed the highest difference in expression level
    Original languageEnglish
    Pages (from-to)571-578
    JournalPhysiologia Plantarum
    Volume118
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Fragaria
    strawberries
    firmness
    Fruit
    Holidays
    cinnamoyl CoA reductase
    Oligonucleotide Array Sequence Analysis
    Gene Expression
    gene expression
    fruits
    cultivars
    Clone Cells
    RNA
    Lignin
    clones
    cinnamyl alcohol dehydrogenase
    Cell Wall
    Genes
    Breeding
    genes

    Keywords

    • cinnamyl alcohol-dehydrogenase
    • plant-cell walls
    • ripening strawberry
    • dna microarrays
    • down-regulation
    • pectate lyase
    • lignin
    • endo-beta-1,4-glucanase
    • manipulation
    • cloning

    Cite this

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    title = "Differential Gene Expression Analysis of Strawberry Cultivars that Differ in Fruit-firmness",
    abstract = "Firmness is an important selection criterium in the breeding of fruit, including strawberry (Fragaria x ananassa Duch.). Clear differences in fruit-firmness are observed between cultivars. In order to identify candidate genes which might be associated with such textural differences, gene expression levels were compared for a soft and a firm cultivar (cv. Gorella and cv. Holiday, respectively). DNA-microarrays representing 1701 strawberry cDNAs were used for simultaneous hybridization of two RNA populations derived from red ripe fruit of both cultivars. In total 61 clones (3.6 f the total cDNAs on the arrays) displayed differential expression, including 10 clones (8 different ones) which showed homology to cell wall related genes in the public databases. The results from the microarray experiments were further confirmed by RNA gel blots, which were also used to examine gene expression in a third cultivar, Elsanta, showing an intermediate texture phenotype (offspring of a cross between Gorella and Holiday). Interestingly, two genes encoding proteins catalysing successive reactions in lignin metabolism (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase) showed the highest difference in expression level",
    keywords = "cinnamyl alcohol-dehydrogenase, plant-cell walls, ripening strawberry, dna microarrays, down-regulation, pectate lyase, lignin, endo-beta-1,4-glucanase, manipulation, cloning",
    author = "E.M.J. Salentijn and A. Aharoni and J.G. Schaart and M.J. Boone and F.A. Krens",
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    Differential Gene Expression Analysis of Strawberry Cultivars that Differ in Fruit-firmness. / Salentijn, E.M.J.; Aharoni, A.; Schaart, J.G.; Boone, M.J.; Krens, F.A.

    In: Physiologia Plantarum, Vol. 118, 2003, p. 571-578.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Differential Gene Expression Analysis of Strawberry Cultivars that Differ in Fruit-firmness

    AU - Salentijn, E.M.J.

    AU - Aharoni, A.

    AU - Schaart, J.G.

    AU - Boone, M.J.

    AU - Krens, F.A.

    PY - 2003

    Y1 - 2003

    N2 - Firmness is an important selection criterium in the breeding of fruit, including strawberry (Fragaria x ananassa Duch.). Clear differences in fruit-firmness are observed between cultivars. In order to identify candidate genes which might be associated with such textural differences, gene expression levels were compared for a soft and a firm cultivar (cv. Gorella and cv. Holiday, respectively). DNA-microarrays representing 1701 strawberry cDNAs were used for simultaneous hybridization of two RNA populations derived from red ripe fruit of both cultivars. In total 61 clones (3.6 f the total cDNAs on the arrays) displayed differential expression, including 10 clones (8 different ones) which showed homology to cell wall related genes in the public databases. The results from the microarray experiments were further confirmed by RNA gel blots, which were also used to examine gene expression in a third cultivar, Elsanta, showing an intermediate texture phenotype (offspring of a cross between Gorella and Holiday). Interestingly, two genes encoding proteins catalysing successive reactions in lignin metabolism (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase) showed the highest difference in expression level

    AB - Firmness is an important selection criterium in the breeding of fruit, including strawberry (Fragaria x ananassa Duch.). Clear differences in fruit-firmness are observed between cultivars. In order to identify candidate genes which might be associated with such textural differences, gene expression levels were compared for a soft and a firm cultivar (cv. Gorella and cv. Holiday, respectively). DNA-microarrays representing 1701 strawberry cDNAs were used for simultaneous hybridization of two RNA populations derived from red ripe fruit of both cultivars. In total 61 clones (3.6 f the total cDNAs on the arrays) displayed differential expression, including 10 clones (8 different ones) which showed homology to cell wall related genes in the public databases. The results from the microarray experiments were further confirmed by RNA gel blots, which were also used to examine gene expression in a third cultivar, Elsanta, showing an intermediate texture phenotype (offspring of a cross between Gorella and Holiday). Interestingly, two genes encoding proteins catalysing successive reactions in lignin metabolism (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase) showed the highest difference in expression level

    KW - cinnamyl alcohol-dehydrogenase

    KW - plant-cell walls

    KW - ripening strawberry

    KW - dna microarrays

    KW - down-regulation

    KW - pectate lyase

    KW - lignin

    KW - endo-beta-1,4-glucanase

    KW - manipulation

    KW - cloning

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    DO - 10.1034/j.1399-3054.2003.00138.x

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    SP - 571

    EP - 578

    JO - Physiologia Plantarum

    JF - Physiologia Plantarum

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