Differential expression of two interferon-y genes in common carp (Cyprinus carpio L.)

H.H. Stolte, H.F.J. Savelkoul, G.F. Wiegertjes, G. Flik, B.M.L. Verburg-van Kemenade

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Abstract

Two interferon gamma (IFN-¿) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-¿-2 shows structural and functional characteristics to other vertebrate IFN-¿ genes and is associated with T-lymphocyte function. Expression profiling shows IFN-¿-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN-¿-1, which is structurally different from IFN-¿-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM+ (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN-¿ genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN-¿-2. This corroborates a genuine T-lymphocyte associated role for IFN-¿-2.
Original languageEnglish
Pages (from-to)1467-1481
Number of pages15
JournalDevelopmental and Comparative Immunology
Volume32
Issue number12
DOIs
Publication statusPublished - 2008

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Keywords

  • ifn-gamma
  • t-cells
  • trypanoplasma-borreli
  • transcription factor
  • sequence alignment
  • teleost fish
  • nitric-oxide
  • multiple
  • bet
  • identification

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