Differential expression of two interferon-y genes in common carp (Cyprinus carpio L.)

H.H. Stolte; H.F.J. Savelkoul; G.F. Wiegertjes; G. Flik; B.M.L. Verburg-van Kemenade

doi:10.1016/j.dci.2008.06.012

Differential expression of two interferon-y genes in common carp (Cyprinus carpio L.)

H.H. Stolte, H.F.J. Savelkoul, G.F. Wiegertjes, G. Flik, B.M.L. Verburg-van Kemenade

Research output: Contribution to journal › Article › Academic › peer-review

96 Citations (Scopus)

Abstract

Two interferon gamma (IFN-¿) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-¿-2 shows structural and functional characteristics to other vertebrate IFN-¿ genes and is associated with T-lymphocyte function. Expression profiling shows IFN-¿-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN-¿-1, which is structurally different from IFN-¿-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM+ (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN-¿ genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN-¿-2. This corroborates a genuine T-lymphocyte associated role for IFN-¿-2.

Original language	English
Pages (from-to)	1467-1481
Number of pages	15
Journal	Developmental and Comparative Immunology
Volume	32
Issue number	12
DOIs	https://doi.org/10.1016/j.dci.2008.06.012
Publication status	Published - 2008

Keywords

ifn-gamma
t-cells
trypanoplasma-borreli
transcription factor
sequence alignment
teleost fish
nitric-oxide
multiple
bet
identification

Access to Document

10.1016/j.dci.2008.06.012

Fingerprint Dive into the research topics of 'Differential expression of two interferon-y genes in common carp (Cyprinus carpio L.)'. Together they form a unique fingerprint.

Cite this

@article{e14ea12840fc4ec69339d25dbe69bbc3,

title = "Differential expression of two interferon-y genes in common carp (Cyprinus carpio L.)",

abstract = "Two interferon gamma (IFN-¿) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-¿-2 shows structural and functional characteristics to other vertebrate IFN-¿ genes and is associated with T-lymphocyte function. Expression profiling shows IFN-¿-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN-¿-1, which is structurally different from IFN-¿-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM+ (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN-¿ genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN-¿-2. This corroborates a genuine T-lymphocyte associated role for IFN-¿-2.",

keywords = "ifn-gamma, t-cells, trypanoplasma-borreli, transcription factor, sequence alignment, teleost fish, nitric-oxide, multiple, bet, identification",

author = "H.H. Stolte and H.F.J. Savelkoul and G.F. Wiegertjes and G. Flik and {Verburg-van Kemenade}, B.M.L.",

year = "2008",

doi = "10.1016/j.dci.2008.06.012",

language = "English",

volume = "32",

pages = "1467--1481",

journal = "Developmental and Comparative Immunology",

issn = "0145-305X",

publisher = "Elsevier",

number = "12",

}

TY - JOUR

T1 - Differential expression of two interferon-y genes in common carp (Cyprinus carpio L.)

AU - Stolte, H.H.

AU - Savelkoul, H.F.J.

AU - Wiegertjes, G.F.

AU - Flik, G.

AU - Verburg-van Kemenade, B.M.L.

PY - 2008

Y1 - 2008

N2 - Two interferon gamma (IFN-¿) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-¿-2 shows structural and functional characteristics to other vertebrate IFN-¿ genes and is associated with T-lymphocyte function. Expression profiling shows IFN-¿-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN-¿-1, which is structurally different from IFN-¿-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM+ (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN-¿ genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN-¿-2. This corroborates a genuine T-lymphocyte associated role for IFN-¿-2.

AB - Two interferon gamma (IFN-¿) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-¿-2 shows structural and functional characteristics to other vertebrate IFN-¿ genes and is associated with T-lymphocyte function. Expression profiling shows IFN-¿-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN-¿-1, which is structurally different from IFN-¿-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM+ (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN-¿ genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN-¿-2. This corroborates a genuine T-lymphocyte associated role for IFN-¿-2.

KW - ifn-gamma

KW - t-cells

KW - trypanoplasma-borreli

KW - transcription factor

KW - sequence alignment

KW - teleost fish

KW - nitric-oxide

KW - multiple

KW - bet

KW - identification

U2 - 10.1016/j.dci.2008.06.012

DO - 10.1016/j.dci.2008.06.012

M3 - Article

VL - 32

SP - 1467

EP - 1481

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 12

ER -