TY - JOUR
T1 - Differential expression of genes involved in C1 metabolism and lignin biosynthesis in wooden core and bast tissues of fibre hemp (Cannabis sativa L.).
AU - van den Broeck, H.C.
AU - Maliepaard, C.A.
AU - Ebskamp, M.J.M.
AU - Toonen, M.A.J.
AU - Koops, A.J.
PY - 2008
Y1 - 2008
N2 - Plants are the major source of fibres for, e.g., textile and paper applications. Fibre hemp (Cannabis sativa L.) can be grown under a wide variety
of agro-ecological conditions, is resistant to weeds and pests and, in general, drought tolerant. Fibre length and content of cellulose and lignin are
important quality parameters for raw material used in cordage, textile, paper, and fibreboard industries. To improve currently used hemp varieties,
more knowledge about the molecular processes that underlie cell wall metabolism is needed. To study gene expression in hemp, separate cDNA
libraries were constructed from developing core fibres and developing and near mature bast fibres. A cDNA microarray was constructed with 3414
randomly selected hemp cDNAs. From these a total number of 1571 sequences was obtained from the 50 ends of the cDNAs. After similarity search,
1036 unique contigs were obtained. To study differences in the genetic background of quality properties of bast and core fibres, bast and core tissues
were obtained from middle parts of the stems during various developmental stages and expression of the cDNAs was analyzed. A total number of
178 clones encoding 65 proteins was found to be more highly expressed in core tissue. Most of these 65 proteins are involved in C1 metabolism and
lignin biosynthesis. In bast tissue, a total number of 110 clones encoding 44 proteins was more highly expressed. Among these 44 proteins are
arabinogalactan proteins, lipid transfer proteins, lipoxygenases, and endoxyloglucan transferases. This paper describes the identification of genes
that are differentially expressed in bast fibres and wooden core tissue of hemp, and is the first step in linking the genetic background to quality
differences between these two types of hemp tissue. This research may result in biotechnological approaches to increase the value of hemp grown
for industrial use
AB - Plants are the major source of fibres for, e.g., textile and paper applications. Fibre hemp (Cannabis sativa L.) can be grown under a wide variety
of agro-ecological conditions, is resistant to weeds and pests and, in general, drought tolerant. Fibre length and content of cellulose and lignin are
important quality parameters for raw material used in cordage, textile, paper, and fibreboard industries. To improve currently used hemp varieties,
more knowledge about the molecular processes that underlie cell wall metabolism is needed. To study gene expression in hemp, separate cDNA
libraries were constructed from developing core fibres and developing and near mature bast fibres. A cDNA microarray was constructed with 3414
randomly selected hemp cDNAs. From these a total number of 1571 sequences was obtained from the 50 ends of the cDNAs. After similarity search,
1036 unique contigs were obtained. To study differences in the genetic background of quality properties of bast and core fibres, bast and core tissues
were obtained from middle parts of the stems during various developmental stages and expression of the cDNAs was analyzed. A total number of
178 clones encoding 65 proteins was found to be more highly expressed in core tissue. Most of these 65 proteins are involved in C1 metabolism and
lignin biosynthesis. In bast tissue, a total number of 110 clones encoding 44 proteins was more highly expressed. Among these 44 proteins are
arabinogalactan proteins, lipid transfer proteins, lipoxygenases, and endoxyloglucan transferases. This paper describes the identification of genes
that are differentially expressed in bast fibres and wooden core tissue of hemp, and is the first step in linking the genetic background to quality
differences between these two types of hemp tissue. This research may result in biotechnological approaches to increase the value of hemp grown
for industrial use
KW - aspen populus-tremuloides
KW - cellulose synthase gene
KW - cell-wall biosynthesis
KW - loblolly-pine
KW - functional genomics
KW - usitatissimum l.
KW - sequence tags
KW - xylem
KW - arabidopsis
KW - lignification
U2 - 10.1016/j.plantsci.2007.11.008
DO - 10.1016/j.plantsci.2007.11.008
M3 - Article
VL - 174
SP - 205
EP - 220
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 2
ER -