Differences between intestinal segments and soybean meal-induced changes in intestinal mucus composition of common carp Cyprinus carpio L.

M.C. van der Marel, M.J. Propsting, F. Battermann, V. Jung-Schroers, A. Hubner, J.H.W.M. Rombout, D. Steinhagen

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

The alimentary tract is a possible site where pathogens and toxins can enter. The alimentary tract is protected, amongst others, by mucus. In this study, tissue samples and crude mucus preparations from different parts of the intestinal tract of Cyprinus carpio (from intestinal bulb onto the hindgut) were examined using histological, histochemical and biochemical techniques. Furthermore, the response of the intestinal mucosal layer and intestinal mucus of C. carpio to acute soybean meal (SBM)-induced enteritis was investigated. In the present study, an indication for a different protein core of mucus high molecular weight glycoproteins (HMGs) for first and second segment could not be found. However, differences in mucus glycosylation could be found. Along the gut axis, the size of the major protein peaks were not similar, which can be caused by a different glycosylation. Also, differences in staining for the antimicrobial peptide beta-defensin 2 were found. Furthermore, changes in HMGs upon SBM diet were found similar to those found in inflammatory bowel disease (IBD) in humans. Initial changes include: changes in mucin composition, the presence of BD3 and of bacteria in internal organs. After the initial changes, all values measured returned back to the initial pre-SBM diet values.
Original languageEnglish
Pages (from-to)12-24
JournalAquaculture Nutrition
Volume20
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • salmon salmo-salar
  • perorally applied endotoxin
  • atlantic salmon
  • aeromonas-hydrophila
  • mucin glycoproteins
  • induced enteritis
  • distal intestine
  • skin mucus
  • in-vivo
  • adhesion

Fingerprint Dive into the research topics of 'Differences between intestinal segments and soybean meal-induced changes in intestinal mucus composition of common carp Cyprinus carpio L.'. Together they form a unique fingerprint.

  • Cite this

    van der Marel, M. C., Propsting, M. J., Battermann, F., Jung-Schroers, V., Hubner, A., Rombout, J. H. W. M., & Steinhagen, D. (2014). Differences between intestinal segments and soybean meal-induced changes in intestinal mucus composition of common carp Cyprinus carpio L. Aquaculture Nutrition, 20(1), 12-24. https://doi.org/10.1111/anu.12041