Difference in the Breast Milk Proteome between Allergic and Non-Allergic Mothers

K.A. Hettinga, F.M. Reina, J.A. Boeren, L. Zhang, G.H. Koppelman, D.S. Postma, J.J.M. Vervoort, A.H. Wijga

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Background Breastfeeding has been linked to a reduction in the prevalence of allergy and asthma. However, studies on this relationship vary in outcome, which may partly be related to differences in breast milk composition. In particular breast milk composition may differ between allergic and non-allergic mothers. Important components that may be involved are breast milk proteins, as these are known to regulate immune development in the newborn. The objective of this study was therefore to explore differences in the proteins of breast milk from 20 allergic and non-allergic mothers. The results from this comparison may then be used to generate hypotheses on proteins associated with allergy in their offspring. Methods Milk samples from allergic and non-allergic mothers were obtained from the PIAMA project, a prospective birth cohort study on incidence, risk factors, and prevention of asthma and inhalant allergy. Non-targeted proteomics technology, based on liquid chromatography and mass spectrometry, was used to compare breast milk from allergic and non-allergic mothers. Results Nineteen proteins, out of a total of 364 proteins identified in both groups, differed significantly in concentration between the breast milk of allergic and non-allergic mothers. Protease inhibitors and apolipoproteins were present in much higher concentrations in breast milk of allergic than non-allergic mothers. These proteins have been suggested to be linked to allergy and asthma. Conclusions The non-targeted milk proteomic analysis employed has provided new targets for future studies on the relation between breast milk composition and allergy.
LanguageEnglish
Article numbere0122234
Number of pages11
JournalPLoS ONE
Volume10
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Human Milk
Proteome
breast milk
proteome
Allergies
hypersensitivity
asthma
milk composition
Hypersensitivity
Asthma
proteins
proteomics
Proteins
Proteomics
milk allergy
Chemical analysis
Milk Hypersensitivity
apolipoproteins
milk proteins
proteinase inhibitors

Cite this

Hettinga, K. A., Reina, F. M., Boeren, J. A., Zhang, L., Koppelman, G. H., Postma, D. S., ... Wijga, A. H. (2015). Difference in the Breast Milk Proteome between Allergic and Non-Allergic Mothers. PLoS ONE, 10(3), [e0122234]. https://doi.org/10.1371/journal.pone.0122234
Hettinga, K.A. ; Reina, F.M. ; Boeren, J.A. ; Zhang, L. ; Koppelman, G.H. ; Postma, D.S. ; Vervoort, J.J.M. ; Wijga, A.H. / Difference in the Breast Milk Proteome between Allergic and Non-Allergic Mothers. In: PLoS ONE. 2015 ; Vol. 10, No. 3.
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abstract = "Background Breastfeeding has been linked to a reduction in the prevalence of allergy and asthma. However, studies on this relationship vary in outcome, which may partly be related to differences in breast milk composition. In particular breast milk composition may differ between allergic and non-allergic mothers. Important components that may be involved are breast milk proteins, as these are known to regulate immune development in the newborn. The objective of this study was therefore to explore differences in the proteins of breast milk from 20 allergic and non-allergic mothers. The results from this comparison may then be used to generate hypotheses on proteins associated with allergy in their offspring. Methods Milk samples from allergic and non-allergic mothers were obtained from the PIAMA project, a prospective birth cohort study on incidence, risk factors, and prevention of asthma and inhalant allergy. Non-targeted proteomics technology, based on liquid chromatography and mass spectrometry, was used to compare breast milk from allergic and non-allergic mothers. Results Nineteen proteins, out of a total of 364 proteins identified in both groups, differed significantly in concentration between the breast milk of allergic and non-allergic mothers. Protease inhibitors and apolipoproteins were present in much higher concentrations in breast milk of allergic than non-allergic mothers. These proteins have been suggested to be linked to allergy and asthma. Conclusions The non-targeted milk proteomic analysis employed has provided new targets for future studies on the relation between breast milk composition and allergy.",
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Difference in the Breast Milk Proteome between Allergic and Non-Allergic Mothers. / Hettinga, K.A.; Reina, F.M.; Boeren, J.A.; Zhang, L.; Koppelman, G.H.; Postma, D.S.; Vervoort, J.J.M.; Wijga, A.H.

In: PLoS ONE, Vol. 10, No. 3, e0122234, 2015.

Research output: Contribution to journalArticleAcademicpeer-review

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N2 - Background Breastfeeding has been linked to a reduction in the prevalence of allergy and asthma. However, studies on this relationship vary in outcome, which may partly be related to differences in breast milk composition. In particular breast milk composition may differ between allergic and non-allergic mothers. Important components that may be involved are breast milk proteins, as these are known to regulate immune development in the newborn. The objective of this study was therefore to explore differences in the proteins of breast milk from 20 allergic and non-allergic mothers. The results from this comparison may then be used to generate hypotheses on proteins associated with allergy in their offspring. Methods Milk samples from allergic and non-allergic mothers were obtained from the PIAMA project, a prospective birth cohort study on incidence, risk factors, and prevention of asthma and inhalant allergy. Non-targeted proteomics technology, based on liquid chromatography and mass spectrometry, was used to compare breast milk from allergic and non-allergic mothers. Results Nineteen proteins, out of a total of 364 proteins identified in both groups, differed significantly in concentration between the breast milk of allergic and non-allergic mothers. Protease inhibitors and apolipoproteins were present in much higher concentrations in breast milk of allergic than non-allergic mothers. These proteins have been suggested to be linked to allergy and asthma. Conclusions The non-targeted milk proteomic analysis employed has provided new targets for future studies on the relation between breast milk composition and allergy.

AB - Background Breastfeeding has been linked to a reduction in the prevalence of allergy and asthma. However, studies on this relationship vary in outcome, which may partly be related to differences in breast milk composition. In particular breast milk composition may differ between allergic and non-allergic mothers. Important components that may be involved are breast milk proteins, as these are known to regulate immune development in the newborn. The objective of this study was therefore to explore differences in the proteins of breast milk from 20 allergic and non-allergic mothers. The results from this comparison may then be used to generate hypotheses on proteins associated with allergy in their offspring. Methods Milk samples from allergic and non-allergic mothers were obtained from the PIAMA project, a prospective birth cohort study on incidence, risk factors, and prevention of asthma and inhalant allergy. Non-targeted proteomics technology, based on liquid chromatography and mass spectrometry, was used to compare breast milk from allergic and non-allergic mothers. Results Nineteen proteins, out of a total of 364 proteins identified in both groups, differed significantly in concentration between the breast milk of allergic and non-allergic mothers. Protease inhibitors and apolipoproteins were present in much higher concentrations in breast milk of allergic than non-allergic mothers. These proteins have been suggested to be linked to allergy and asthma. Conclusions The non-targeted milk proteomic analysis employed has provided new targets for future studies on the relation between breast milk composition and allergy.

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