Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata)

Leonardo J. Magnoni, Juan Antonio Martos-Sitcha, Augusto Queiroz, Josep Alvar Calduch-Giner, Jose Fernando Magalhaes Gonçalves, Cristina M.R. Rocha, Helena T. Abreu, Johan W. Schrama, Rodrigo O.A. Ozorio*, Jaume Perez-Sanchez

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)

Abstract

Intensive aquaculture practices involve rearing fish at high densities. In these conditions, fish may be exposed to suboptimal dissolved O2 levels with an increased formation of reactive O2 species (ROS) in tissues. Seaweeds (SW) contain biologically active substances with efficient antioxidant capacities. This study evaluated the effects of dietary supplementation of heat-treated SW (5% Gracilaria vermiculophylla or 5% Ulva lactuca) on stress bioindicators in sea bream subjected to a hypoxic challenge. 168 fish (104.5 g average weight) were distributed in 24 tanks, in which eight tanks were fed one of three experimental diets for 34 days: (i) a control diet without SW supplementation, (ii) a control diet supplemented with Ulva, or (iii) a control diet with Gracilaria. Thereafter, fish from 12 tanks (n=4 tanks/dietary treatment) were subjected to 24 h hypoxia (1.3 mg O2 l-1) and subsequent recovery normoxia (8.6 mg O2 l-1). Hypoxic fish showed an increase in hematocrit values regardless of dietary treatment. Dietary modulation of the O2-carrying capacity was conspicuous during recovery, as fish fed SW supplemented diets displayed significantly higher haemoglobin concentration than fish fed the control diet. After the challenge, survival rates in both groups of fish fed SW were higher, which was consistent with a decrease in hepatic lipid peroxidation in these groups. Furthermore, the hepatic antioxidant enzyme activities were modulated differently by changes in environmental O2 condition, particularly in sea bream fed the Gracilaria diet. After being subjected to hypoxia, the gene expression of antioxidant enzymes and molecular chaperones in liver and heart were down regulated in sea bream fed SW diets. This study suggests that the antioxidant properties of heat-treated SW may have a protective role against oxidative stress. The nature of these compounds and possible mechanisms implied are currently being investigated.

Original languageEnglish
Pages (from-to)897-908
JournalBiology Open
Volume6
Issue number6
DOIs
Publication statusPublished - 2017

Fingerprint

Gracilaria
Ulva
Sea Bream
Seaweed
edible seaweed
Sparus aurata
bream
Nutrition
Dietary Supplements
Fish
dietary supplements
hypoxia
Fishes
Hot Temperature
Diet
heat
diet
fish feeds
Antioxidants
antioxidants

Keywords

  • Hypoxia
  • Nutritional background
  • Oxidative stress
  • Sea bream
  • Seaweeds

Cite this

Magnoni, L. J., Martos-Sitcha, J. A., Queiroz, A., Calduch-Giner, J. A., Gonçalves, J. F. M., Rocha, C. M. R., ... Perez-Sanchez, J. (2017). Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata). Biology Open, 6(6), 897-908. https://doi.org/10.1242/bio.024299
Magnoni, Leonardo J. ; Martos-Sitcha, Juan Antonio ; Queiroz, Augusto ; Calduch-Giner, Josep Alvar ; Gonçalves, Jose Fernando Magalhaes ; Rocha, Cristina M.R. ; Abreu, Helena T. ; Schrama, Johan W. ; Ozorio, Rodrigo O.A. ; Perez-Sanchez, Jaume. / Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata). In: Biology Open. 2017 ; Vol. 6, No. 6. pp. 897-908.
@article{544e7de8ef7a485c8fd1f448f575a6a3,
title = "Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata)",
abstract = "Intensive aquaculture practices involve rearing fish at high densities. In these conditions, fish may be exposed to suboptimal dissolved O2 levels with an increased formation of reactive O2 species (ROS) in tissues. Seaweeds (SW) contain biologically active substances with efficient antioxidant capacities. This study evaluated the effects of dietary supplementation of heat-treated SW (5{\%} Gracilaria vermiculophylla or 5{\%} Ulva lactuca) on stress bioindicators in sea bream subjected to a hypoxic challenge. 168 fish (104.5 g average weight) were distributed in 24 tanks, in which eight tanks were fed one of three experimental diets for 34 days: (i) a control diet without SW supplementation, (ii) a control diet supplemented with Ulva, or (iii) a control diet with Gracilaria. Thereafter, fish from 12 tanks (n=4 tanks/dietary treatment) were subjected to 24 h hypoxia (1.3 mg O2 l-1) and subsequent recovery normoxia (8.6 mg O2 l-1). Hypoxic fish showed an increase in hematocrit values regardless of dietary treatment. Dietary modulation of the O2-carrying capacity was conspicuous during recovery, as fish fed SW supplemented diets displayed significantly higher haemoglobin concentration than fish fed the control diet. After the challenge, survival rates in both groups of fish fed SW were higher, which was consistent with a decrease in hepatic lipid peroxidation in these groups. Furthermore, the hepatic antioxidant enzyme activities were modulated differently by changes in environmental O2 condition, particularly in sea bream fed the Gracilaria diet. After being subjected to hypoxia, the gene expression of antioxidant enzymes and molecular chaperones in liver and heart were down regulated in sea bream fed SW diets. This study suggests that the antioxidant properties of heat-treated SW may have a protective role against oxidative stress. The nature of these compounds and possible mechanisms implied are currently being investigated.",
keywords = "Hypoxia, Nutritional background, Oxidative stress, Sea bream, Seaweeds",
author = "Magnoni, {Leonardo J.} and Martos-Sitcha, {Juan Antonio} and Augusto Queiroz and Calduch-Giner, {Josep Alvar} and Gon{\cc}alves, {Jose Fernando Magalhaes} and Rocha, {Cristina M.R.} and Abreu, {Helena T.} and Schrama, {Johan W.} and Ozorio, {Rodrigo O.A.} and Jaume Perez-Sanchez",
year = "2017",
doi = "10.1242/bio.024299",
language = "English",
volume = "6",
pages = "897--908",
journal = "Biology Open",
issn = "2046-6390",
publisher = "Company of Biologists",
number = "6",

}

Magnoni, LJ, Martos-Sitcha, JA, Queiroz, A, Calduch-Giner, JA, Gonçalves, JFM, Rocha, CMR, Abreu, HT, Schrama, JW, Ozorio, ROA & Perez-Sanchez, J 2017, 'Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata)', Biology Open, vol. 6, no. 6, pp. 897-908. https://doi.org/10.1242/bio.024299

Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata). / Magnoni, Leonardo J.; Martos-Sitcha, Juan Antonio; Queiroz, Augusto; Calduch-Giner, Josep Alvar; Gonçalves, Jose Fernando Magalhaes; Rocha, Cristina M.R.; Abreu, Helena T.; Schrama, Johan W.; Ozorio, Rodrigo O.A.; Perez-Sanchez, Jaume.

In: Biology Open, Vol. 6, No. 6, 2017, p. 897-908.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead sea bream (Sparus aurata)

AU - Magnoni, Leonardo J.

AU - Martos-Sitcha, Juan Antonio

AU - Queiroz, Augusto

AU - Calduch-Giner, Josep Alvar

AU - Gonçalves, Jose Fernando Magalhaes

AU - Rocha, Cristina M.R.

AU - Abreu, Helena T.

AU - Schrama, Johan W.

AU - Ozorio, Rodrigo O.A.

AU - Perez-Sanchez, Jaume

PY - 2017

Y1 - 2017

N2 - Intensive aquaculture practices involve rearing fish at high densities. In these conditions, fish may be exposed to suboptimal dissolved O2 levels with an increased formation of reactive O2 species (ROS) in tissues. Seaweeds (SW) contain biologically active substances with efficient antioxidant capacities. This study evaluated the effects of dietary supplementation of heat-treated SW (5% Gracilaria vermiculophylla or 5% Ulva lactuca) on stress bioindicators in sea bream subjected to a hypoxic challenge. 168 fish (104.5 g average weight) were distributed in 24 tanks, in which eight tanks were fed one of three experimental diets for 34 days: (i) a control diet without SW supplementation, (ii) a control diet supplemented with Ulva, or (iii) a control diet with Gracilaria. Thereafter, fish from 12 tanks (n=4 tanks/dietary treatment) were subjected to 24 h hypoxia (1.3 mg O2 l-1) and subsequent recovery normoxia (8.6 mg O2 l-1). Hypoxic fish showed an increase in hematocrit values regardless of dietary treatment. Dietary modulation of the O2-carrying capacity was conspicuous during recovery, as fish fed SW supplemented diets displayed significantly higher haemoglobin concentration than fish fed the control diet. After the challenge, survival rates in both groups of fish fed SW were higher, which was consistent with a decrease in hepatic lipid peroxidation in these groups. Furthermore, the hepatic antioxidant enzyme activities were modulated differently by changes in environmental O2 condition, particularly in sea bream fed the Gracilaria diet. After being subjected to hypoxia, the gene expression of antioxidant enzymes and molecular chaperones in liver and heart were down regulated in sea bream fed SW diets. This study suggests that the antioxidant properties of heat-treated SW may have a protective role against oxidative stress. The nature of these compounds and possible mechanisms implied are currently being investigated.

AB - Intensive aquaculture practices involve rearing fish at high densities. In these conditions, fish may be exposed to suboptimal dissolved O2 levels with an increased formation of reactive O2 species (ROS) in tissues. Seaweeds (SW) contain biologically active substances with efficient antioxidant capacities. This study evaluated the effects of dietary supplementation of heat-treated SW (5% Gracilaria vermiculophylla or 5% Ulva lactuca) on stress bioindicators in sea bream subjected to a hypoxic challenge. 168 fish (104.5 g average weight) were distributed in 24 tanks, in which eight tanks were fed one of three experimental diets for 34 days: (i) a control diet without SW supplementation, (ii) a control diet supplemented with Ulva, or (iii) a control diet with Gracilaria. Thereafter, fish from 12 tanks (n=4 tanks/dietary treatment) were subjected to 24 h hypoxia (1.3 mg O2 l-1) and subsequent recovery normoxia (8.6 mg O2 l-1). Hypoxic fish showed an increase in hematocrit values regardless of dietary treatment. Dietary modulation of the O2-carrying capacity was conspicuous during recovery, as fish fed SW supplemented diets displayed significantly higher haemoglobin concentration than fish fed the control diet. After the challenge, survival rates in both groups of fish fed SW were higher, which was consistent with a decrease in hepatic lipid peroxidation in these groups. Furthermore, the hepatic antioxidant enzyme activities were modulated differently by changes in environmental O2 condition, particularly in sea bream fed the Gracilaria diet. After being subjected to hypoxia, the gene expression of antioxidant enzymes and molecular chaperones in liver and heart were down regulated in sea bream fed SW diets. This study suggests that the antioxidant properties of heat-treated SW may have a protective role against oxidative stress. The nature of these compounds and possible mechanisms implied are currently being investigated.

KW - Hypoxia

KW - Nutritional background

KW - Oxidative stress

KW - Sea bream

KW - Seaweeds

U2 - 10.1242/bio.024299

DO - 10.1242/bio.024299

M3 - Article

VL - 6

SP - 897

EP - 908

JO - Biology Open

JF - Biology Open

SN - 2046-6390

IS - 6

ER -