Dietary electrolyte balance affects growth performance, amylase activity and metabolic response in the meagre (Argyrosomus regius)

Leonardo J. Magnoni*, Emilio Salas-Leiton, Maria João Peixoto, Luis Pereira, Francisca Silva-Brito, Filipa Fontinha, José F.M. Gonçalves, Jonathan M. Wilson, Johan W. Schrama, Rodrigo O.A. Ozório

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Dietary ion content is known to alter the acid-base balance in freshwater fish. The current study investigated the metabolic impact of acid-base disturbances produced by differences in dietary electrolyte balance (DEB) in the meagre (Argyrosomus regius), an euryhaline species. Changes in fish performance, gastric chyme characteristics, pH and ion concentrations in the bloodstream, digestive enzyme activities and metabolic rates were analyzed in meagre fed ad libitum two experimental diets (DEB 200 or DEB 700mEq/kg) differing in the Na2CO3 content for 69days. Fish fed the DEB 200 diet had 60-66% better growth performance than the DEB 700 group. Meagre consuming the DEB 200 diet were 90-96% more efficient than fish fed the DEB 700 diet at allocating energy from feed into somatic growth. The pH values in blood were significantly lower in the DEB 700 group 2h after feeding when compared to DEB 200, indicating that acid-base balance in meagre was affected by electrolyte balance in diet. Osmolality, and Na+ and K+ concentrations in plasma did not vary with the dietary treatment. Gastric chyme in the DEB 700 group had higher pH values, dry matter, protein and energy contents, but lower lipid content than in the DEB 200 group. Twenty-four hours after feeding, amylase activity was higher in the gastrointestinal tract of DEB 700 group when compared to the DEB 200 group. DEB 700 group had lower routine metabolic (RMR) and standard metabolic (SMR) rates, indicating a decrease in maintenance energy expenditure 48h after feeding the alkaline diet. The current study demonstrates that feeding meagre with an alkaline diet not only causes acid-base imbalance, but also negatively affects digestion and possibly nutrient assimilation, resulting in decreased growth performance.

Original languageEnglish
Pages (from-to)8-15
JournalComparative Biochemistry and Physiology. B, Biochemistry and Molecular Biology
Volume211
DOIs
Publication statusPublished - 2017

Fingerprint

Water-Electrolyte Balance
Amylases
Electrolytes
Growth
Nutrition
Diet
Fish
Fishes
Acid-Base Equilibrium
Stomach
Acid-Base Imbalance
Ions
Acids
Enzyme activity
Fresh Water
Osmolar Concentration
Energy Metabolism
Nutrients

Keywords

  • Acid-base balance
  • Dietary cation-anion difference
  • Digestion
  • Energy use
  • Euryhaline fish

Cite this

Magnoni, Leonardo J. ; Salas-Leiton, Emilio ; Peixoto, Maria João ; Pereira, Luis ; Silva-Brito, Francisca ; Fontinha, Filipa ; Gonçalves, José F.M. ; Wilson, Jonathan M. ; Schrama, Johan W. ; Ozório, Rodrigo O.A. / Dietary electrolyte balance affects growth performance, amylase activity and metabolic response in the meagre (Argyrosomus regius). In: Comparative Biochemistry and Physiology. B, Biochemistry and Molecular Biology. 2017 ; Vol. 211. pp. 8-15.
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abstract = "Dietary ion content is known to alter the acid-base balance in freshwater fish. The current study investigated the metabolic impact of acid-base disturbances produced by differences in dietary electrolyte balance (DEB) in the meagre (Argyrosomus regius), an euryhaline species. Changes in fish performance, gastric chyme characteristics, pH and ion concentrations in the bloodstream, digestive enzyme activities and metabolic rates were analyzed in meagre fed ad libitum two experimental diets (DEB 200 or DEB 700mEq/kg) differing in the Na2CO3 content for 69days. Fish fed the DEB 200 diet had 60-66{\%} better growth performance than the DEB 700 group. Meagre consuming the DEB 200 diet were 90-96{\%} more efficient than fish fed the DEB 700 diet at allocating energy from feed into somatic growth. The pH values in blood were significantly lower in the DEB 700 group 2h after feeding when compared to DEB 200, indicating that acid-base balance in meagre was affected by electrolyte balance in diet. Osmolality, and Na+ and K+ concentrations in plasma did not vary with the dietary treatment. Gastric chyme in the DEB 700 group had higher pH values, dry matter, protein and energy contents, but lower lipid content than in the DEB 200 group. Twenty-four hours after feeding, amylase activity was higher in the gastrointestinal tract of DEB 700 group when compared to the DEB 200 group. DEB 700 group had lower routine metabolic (RMR) and standard metabolic (SMR) rates, indicating a decrease in maintenance energy expenditure 48h after feeding the alkaline diet. The current study demonstrates that feeding meagre with an alkaline diet not only causes acid-base imbalance, but also negatively affects digestion and possibly nutrient assimilation, resulting in decreased growth performance.",
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Dietary electrolyte balance affects growth performance, amylase activity and metabolic response in the meagre (Argyrosomus regius). / Magnoni, Leonardo J.; Salas-Leiton, Emilio; Peixoto, Maria João; Pereira, Luis; Silva-Brito, Francisca; Fontinha, Filipa; Gonçalves, José F.M.; Wilson, Jonathan M.; Schrama, Johan W.; Ozório, Rodrigo O.A.

In: Comparative Biochemistry and Physiology. B, Biochemistry and Molecular Biology, Vol. 211, 2017, p. 8-15.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Dietary electrolyte balance affects growth performance, amylase activity and metabolic response in the meagre (Argyrosomus regius)

AU - Magnoni, Leonardo J.

AU - Salas-Leiton, Emilio

AU - Peixoto, Maria João

AU - Pereira, Luis

AU - Silva-Brito, Francisca

AU - Fontinha, Filipa

AU - Gonçalves, José F.M.

AU - Wilson, Jonathan M.

AU - Schrama, Johan W.

AU - Ozório, Rodrigo O.A.

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N2 - Dietary ion content is known to alter the acid-base balance in freshwater fish. The current study investigated the metabolic impact of acid-base disturbances produced by differences in dietary electrolyte balance (DEB) in the meagre (Argyrosomus regius), an euryhaline species. Changes in fish performance, gastric chyme characteristics, pH and ion concentrations in the bloodstream, digestive enzyme activities and metabolic rates were analyzed in meagre fed ad libitum two experimental diets (DEB 200 or DEB 700mEq/kg) differing in the Na2CO3 content for 69days. Fish fed the DEB 200 diet had 60-66% better growth performance than the DEB 700 group. Meagre consuming the DEB 200 diet were 90-96% more efficient than fish fed the DEB 700 diet at allocating energy from feed into somatic growth. The pH values in blood were significantly lower in the DEB 700 group 2h after feeding when compared to DEB 200, indicating that acid-base balance in meagre was affected by electrolyte balance in diet. Osmolality, and Na+ and K+ concentrations in plasma did not vary with the dietary treatment. Gastric chyme in the DEB 700 group had higher pH values, dry matter, protein and energy contents, but lower lipid content than in the DEB 200 group. Twenty-four hours after feeding, amylase activity was higher in the gastrointestinal tract of DEB 700 group when compared to the DEB 200 group. DEB 700 group had lower routine metabolic (RMR) and standard metabolic (SMR) rates, indicating a decrease in maintenance energy expenditure 48h after feeding the alkaline diet. The current study demonstrates that feeding meagre with an alkaline diet not only causes acid-base imbalance, but also negatively affects digestion and possibly nutrient assimilation, resulting in decreased growth performance.

AB - Dietary ion content is known to alter the acid-base balance in freshwater fish. The current study investigated the metabolic impact of acid-base disturbances produced by differences in dietary electrolyte balance (DEB) in the meagre (Argyrosomus regius), an euryhaline species. Changes in fish performance, gastric chyme characteristics, pH and ion concentrations in the bloodstream, digestive enzyme activities and metabolic rates were analyzed in meagre fed ad libitum two experimental diets (DEB 200 or DEB 700mEq/kg) differing in the Na2CO3 content for 69days. Fish fed the DEB 200 diet had 60-66% better growth performance than the DEB 700 group. Meagre consuming the DEB 200 diet were 90-96% more efficient than fish fed the DEB 700 diet at allocating energy from feed into somatic growth. The pH values in blood were significantly lower in the DEB 700 group 2h after feeding when compared to DEB 200, indicating that acid-base balance in meagre was affected by electrolyte balance in diet. Osmolality, and Na+ and K+ concentrations in plasma did not vary with the dietary treatment. Gastric chyme in the DEB 700 group had higher pH values, dry matter, protein and energy contents, but lower lipid content than in the DEB 200 group. Twenty-four hours after feeding, amylase activity was higher in the gastrointestinal tract of DEB 700 group when compared to the DEB 200 group. DEB 700 group had lower routine metabolic (RMR) and standard metabolic (SMR) rates, indicating a decrease in maintenance energy expenditure 48h after feeding the alkaline diet. The current study demonstrates that feeding meagre with an alkaline diet not only causes acid-base imbalance, but also negatively affects digestion and possibly nutrient assimilation, resulting in decreased growth performance.

KW - Acid-base balance

KW - Dietary cation-anion difference

KW - Digestion

KW - Energy use

KW - Euryhaline fish

U2 - 10.1016/j.cbpb.2017.03.001

DO - 10.1016/j.cbpb.2017.03.001

M3 - Article

VL - 211

SP - 8

EP - 15

JO - Comparative Biochemistry and Physiology. B, Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology. B, Biochemistry and Molecular Biology

SN - 1096-4959

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