Dietary advanced glycation endproducts induce an inflammatory response in human macrophages in vitro

Timme van der Lugt, Antje R. Weseler, Wouter A. Gebbink, Misha F. Vrolijk, Antoon Opperhuizen, Aalt Bast

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Advanced glycation endproducts (AGEs) can be found in protein-and sugar-rich food products processed at high temperatures, which make up a vast amount of the Western diet. The effect of AGE-rich food products on human health is not yet clear and controversy still exists due to possible contamination of samples with endotoxin and the use of endogenous formed AGEs. AGEs occur in food products, both as protein-bound and individual molecules. Which form exactly induces a pro-inflammatory effect is also unknown. In this study, we exposed human macrophage-like cells to dietary AGEs, both in a protein matrix and individual AGEs. It was ensured that all samples did not contain endotoxin concentrations > 0.06 EU/mL. The dietary AGEs induced TNF-alpha secretion of human macrophage-like cells. This effect was decreased by the addition of N(ε)-carboxymethyllysine (CML)-antibodies or a receptor for advanced glycation endproducts (RAGE) antagonist. None of the individual AGEs induce any TNF-alpha, indicating that AGEs should be bound to proteins to exert an inflammatory reaction. These findings show that dietary AGEs directly stimulate the inflammatory response of human innate immune cells and help us define the risk of regular consumption of AGE-rich food products on human health.

Original languageEnglish
Article number1868
JournalNutrients
Volume10
Issue number12
DOIs
Publication statusPublished - 2 Dec 2018

Fingerprint

macrophages
inflammation
Macrophages
Food
Endotoxins
Proteins
Tumor Necrosis Factor-alpha
foods
Health
Innate Immunity
endotoxins
tumor necrosis factor-alpha
human health
In Vitro Techniques
advanced glycation end products
proteins
Temperature
Antibodies
cells
antagonists

Keywords

  • Advanced glycation endproducts
  • Food
  • Inflammation
  • Macrophages
  • Maillard reaction

Cite this

van der Lugt, T., Weseler, A. R., Gebbink, W. A., Vrolijk, M. F., Opperhuizen, A., & Bast, A. (2018). Dietary advanced glycation endproducts induce an inflammatory response in human macrophages in vitro. Nutrients, 10(12), [1868]. https://doi.org/10.3390/nu10121868
van der Lugt, Timme ; Weseler, Antje R. ; Gebbink, Wouter A. ; Vrolijk, Misha F. ; Opperhuizen, Antoon ; Bast, Aalt. / Dietary advanced glycation endproducts induce an inflammatory response in human macrophages in vitro. In: Nutrients. 2018 ; Vol. 10, No. 12.
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van der Lugt, T, Weseler, AR, Gebbink, WA, Vrolijk, MF, Opperhuizen, A & Bast, A 2018, 'Dietary advanced glycation endproducts induce an inflammatory response in human macrophages in vitro', Nutrients, vol. 10, no. 12, 1868. https://doi.org/10.3390/nu10121868

Dietary advanced glycation endproducts induce an inflammatory response in human macrophages in vitro. / van der Lugt, Timme; Weseler, Antje R.; Gebbink, Wouter A.; Vrolijk, Misha F.; Opperhuizen, Antoon; Bast, Aalt.

In: Nutrients, Vol. 10, No. 12, 1868, 02.12.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Dietary advanced glycation endproducts induce an inflammatory response in human macrophages in vitro

AU - van der Lugt, Timme

AU - Weseler, Antje R.

AU - Gebbink, Wouter A.

AU - Vrolijk, Misha F.

AU - Opperhuizen, Antoon

AU - Bast, Aalt

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N2 - Advanced glycation endproducts (AGEs) can be found in protein-and sugar-rich food products processed at high temperatures, which make up a vast amount of the Western diet. The effect of AGE-rich food products on human health is not yet clear and controversy still exists due to possible contamination of samples with endotoxin and the use of endogenous formed AGEs. AGEs occur in food products, both as protein-bound and individual molecules. Which form exactly induces a pro-inflammatory effect is also unknown. In this study, we exposed human macrophage-like cells to dietary AGEs, both in a protein matrix and individual AGEs. It was ensured that all samples did not contain endotoxin concentrations > 0.06 EU/mL. The dietary AGEs induced TNF-alpha secretion of human macrophage-like cells. This effect was decreased by the addition of N(ε)-carboxymethyllysine (CML)-antibodies or a receptor for advanced glycation endproducts (RAGE) antagonist. None of the individual AGEs induce any TNF-alpha, indicating that AGEs should be bound to proteins to exert an inflammatory reaction. These findings show that dietary AGEs directly stimulate the inflammatory response of human innate immune cells and help us define the risk of regular consumption of AGE-rich food products on human health.

AB - Advanced glycation endproducts (AGEs) can be found in protein-and sugar-rich food products processed at high temperatures, which make up a vast amount of the Western diet. The effect of AGE-rich food products on human health is not yet clear and controversy still exists due to possible contamination of samples with endotoxin and the use of endogenous formed AGEs. AGEs occur in food products, both as protein-bound and individual molecules. Which form exactly induces a pro-inflammatory effect is also unknown. In this study, we exposed human macrophage-like cells to dietary AGEs, both in a protein matrix and individual AGEs. It was ensured that all samples did not contain endotoxin concentrations > 0.06 EU/mL. The dietary AGEs induced TNF-alpha secretion of human macrophage-like cells. This effect was decreased by the addition of N(ε)-carboxymethyllysine (CML)-antibodies or a receptor for advanced glycation endproducts (RAGE) antagonist. None of the individual AGEs induce any TNF-alpha, indicating that AGEs should be bound to proteins to exert an inflammatory reaction. These findings show that dietary AGEs directly stimulate the inflammatory response of human innate immune cells and help us define the risk of regular consumption of AGE-rich food products on human health.

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KW - Food

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JF - Nutrients

SN - 2072-6643

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ER -

van der Lugt T, Weseler AR, Gebbink WA, Vrolijk MF, Opperhuizen A, Bast A. Dietary advanced glycation endproducts induce an inflammatory response in human macrophages in vitro. Nutrients. 2018 Dec 2;10(12). 1868. https://doi.org/10.3390/nu10121868