Diagnosis of the first cases of scrapie in Poland

M.P. Polak, M. Larska, J.P.M. Langeveld, A. Buschmann, M.H. Groschup, J.F. Zmudzinski

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

This is the first report of cases of scrapie in Poland. The disease was an atypical phenotype, diagnosed in two aged sheep which were found dead. Brainstem samples from both animals were positive on the applied ELISA rapid test, while the confirmatory immunoblot indicated abnormal banding patterns of protease resistant prion protein (PrPres). The genotypes of these sheep were ALRQ/ALHQ and ALRQ/ALRR. The absence of premonitory clinical signs, the advanced age of the affected sheep, the higher concentration of PrPres in the cerebellum relative to the obex, the unusual banding profile of the prion protein and its relatively low resistance to proteolytic degradation confirmed the diagnosis of atypical scrapie (Nor98-like) in both cases
Original languageEnglish
Pages (from-to)47-52
JournalThe Veterinary Journal
Volume186
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • bovine spongiform encephalopathy
  • atypical scrapie
  • prion protein
  • monoclonal-antibodies
  • prp genotypes
  • sheep
  • nor98
  • discrimination
  • strains
  • norway

Fingerprint Dive into the research topics of 'Diagnosis of the first cases of scrapie in Poland'. Together they form a unique fingerprint.

  • Cite this

    Polak, M. P., Larska, M., Langeveld, J. P. M., Buschmann, A., Groschup, M. H., & Zmudzinski, J. F. (2010). Diagnosis of the first cases of scrapie in Poland. The Veterinary Journal, 186(1), 47-52. https://doi.org/10.1016/j.tvjl.2009.07.032