Cereals, especially wheat, may cause several food-related diseases, of which gluten intolerance (coeliac disease, CD) is the best defined: specific immunogenic epitopes, nine amino acid-long peptide sequences, have been identified from various gluten proteins. These may activate T cells, causing inflammation of the small intestine and a wide variety of other symptoms. Here, we review several breeding-related strategies aiming at reduction or elimination of such epitopes from wheat, including variety selection, re-synthesis of hexaploids, deletion of specific chromosomal fragments, RNA-interference, mutagenesis and genome editing using CRISPR/Cas9. The related issue of genetic modification (GM) is discussed. These strategies should lead to wheat food products to be used in gluten-free diets for diagnosed CD individuals and/or to strongly reduce the burden from immunogenic gluten to the non-diagnosed CD population.
|Title of host publication||Proceedings of the 28th meeting of the Working Group on Prolamin Analysis and Toxicity|
|Place of Publication||Nantes, France|
|Publication status||Published - 2014|
|Event||The 28th meeting of the Working Group on Prolamin Analysis and Toxicity - |
Duration: 25 Jul 2014 → 27 Jul 2014
|Conference||The 28th meeting of the Working Group on Prolamin Analysis and Toxicity|
|Period||25/07/14 → 27/07/14|