Development of wheat varieties with reduced contents of celiac-immunogenic epitopes through conventional and GM strategies

M.J.M. Smulders, A.A. Jouanin, J.G. Schaart, R.G.F. Visser, J. Cockram, F. Leigh, E. Wallington, L.A. Boyd, H.C. van den Broeck, I.M. van der Meer, L.J.W.J. Gilissen

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademicpeer-review

Abstract

Cereals, especially wheat, may cause several food-related diseases, of which gluten intolerance (coeliac disease, CD) is the best defined: specific immunogenic epitopes, nine amino acid-long peptide sequences, have been identified from various gluten proteins. These may activate T cells, causing inflammation of the small intestine and a wide variety of other symptoms. Here, we review several breeding-related strategies aiming at reduction or elimination of such epitopes from wheat, including variety selection, re-synthesis of hexaploids, deletion of specific chromosomal fragments, RNA-interference, mutagenesis and genome editing using CRISPR/Cas9. The related issue of genetic modification (GM) is discussed. These strategies should lead to wheat food products to be used in gluten-free diets for diagnosed CD individuals and/or to strongly reduce the burden from immunogenic gluten to the non-diagnosed CD population.
Original languageEnglish
Title of host publicationProceedings of the 28th meeting of the Working Group on Prolamin Analysis and Toxicity
EditorsP. Koehler
Place of PublicationNantes, France
PublisherWGPAT
Pages47-56
ISBN (Print)9783938896921
Publication statusPublished - 2014
EventThe 28th meeting of the Working Group on Prolamin Analysis and Toxicity -
Duration: 25 Jul 201427 Jul 2014

Conference

ConferenceThe 28th meeting of the Working Group on Prolamin Analysis and Toxicity
Period25/07/1427/07/14

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