Development of the WagRhSNP AXIOM SNP Array Based on Sequences from Tetraploid Cut Roses and Garden Roses

M.J.M. Smulders, R.E. Voorrips, G. Esselink, T.M. Santos Leonardo, W.P.C. van 't Westende, M. Vukosavljev, C.F.S. Koning-Boucoiran, W.E. van de Weg, P.F.P. Arens, D. Schulz, T. Debener, L. Bellon, M. Mittmann, A. Pirani, T. Webster, F. Brew, P. Cox, C.A. Maliepaard

Research output: Chapter in Book/Report/Conference proceedingConference paper

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Abstract

Rose, as many other important ornamental, vegetable and field crops, is polyploid. This poses constraints in genetic analyses due to the occurrence of multiple alleles at marker and trait loci and the existence of multiple allele dosages. Developments in marker discovery (next generation sequencing), detection (SNP arrays) and analysis (software for dosage scoring) now make it feasible to develop high-density molecular marker maps for the homologous chromosomes in tetraploids separately, and thus perform QTL analysis in tetraploids. We developed a SNP array for rose to develop genetic maps in tetraploid garden roses and cut roses, which are to be used for inheritance studies and genetic mapping. Here we have indicated the general strategy followed for developing a SNP array and for scoring and using the SNP data generated, and elaborated on the activities undertaken to use the WagRhSNP Axiom array in rose. The array design is not proprietary but can be used by all researchers working in rose
Original languageEnglish
Title of host publicationProceedings VIth International Symposiul on Rose Research and Cultivation
EditorsT. Debener, M. Linde
PublisherISHS
Pages177-184
Volume1064
ISBN (Print)9789462610552
DOIs
Publication statusPublished - 2015
EventVI International Symposiul on Rose Research and Cultivation, Hannover, Germany -
Duration: 25 Aug 201330 Aug 2013

Conference

ConferenceVI International Symposiul on Rose Research and Cultivation, Hannover, Germany
Period25/08/1330/08/13

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    Smulders, M. J. M., Voorrips, R. E., Esselink, G., Santos Leonardo, T. M., van 't Westende, W. P. C., Vukosavljev, M., Koning-Boucoiran, C. F. S., van de Weg, W. E., Arens, P. F. P., Schulz, D., Debener, T., Bellon, L., Mittmann, M., Pirani, A., Webster, T., Brew, F., Cox, P., & Maliepaard, C. A. (2015). Development of the WagRhSNP AXIOM SNP Array Based on Sequences from Tetraploid Cut Roses and Garden Roses. In T. Debener, & M. Linde (Eds.), Proceedings VIth International Symposiul on Rose Research and Cultivation (Vol. 1064, pp. 177-184). ISHS. https://doi.org/10.17660/ActaHortic.2015.1064.20