Some aspects of root nodule development of Pisum sativum inoculated with Rhizobium leguminosarum were examined. 1. Nitrogenase activity (measured as acetylene reduction) appears to be preceded by leghemoglobin synthesis (measured immunologically). 2. Syntheses of component I and component II of nitrogenase are not strictly coordinated. Synthesis of component I starts before component II. 3. Plant and bacteroid protein synthesis (measured by [35S]sulfate labeling) in root nodules declines rapidly during nodule development. Corresponding with this decline is a decrease in quantity and quality of rRNA.
|Journal||Biochimica et biophysica acta. Nucleic acids and protein synthesis|
|Publication status||Published - 1979|
Bisseling, T., van den Bos, R. C., Weststrate, M. W., Hakkaart, M. J. J., & van Kammen, A. (1979). Development of the nitrogen-fixing and protein-synthesizing apparatus of bacteroids in pea root nodules. Biochimica et biophysica acta. Nucleic acids and protein synthesis, 562(3), 515-526.