Development of polymorphic microsatellite markers for the Eucalyptus leaf pathogen Mycosphaerella nubilosa

G.C. Hunter; M.N. Cortinas; B.D. Wingfield; P.W. Crous; M.J. Wingfield

doi:10.1111/j.1471-8286.2006.01392.x

Development of polymorphic microsatellite markers for the Eucalyptus leaf pathogen Mycosphaerella nubilosa

G.C. Hunter, M.N. Cortinas, B.D. Wingfield, P.W. Crous, M.J. Wingfield

Laboratory of Phytopathology

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Mycosphaerella nubilosa is one of the most important Eucalyptus leaf pathogens, causing premature defoliation and stunting of growth. The aim of this study was to develop polymorphic microsatellite markers for M. nubilosa. Fifteen primer sets were developed and evaluated for polymorphism. Two primers were monomorphic, three primers did not amplify the desired region and 10 primer pairs were polymorphic. These microsatellite markers will be applied to population biology studies of M. nubilosa collections from several countries. These studies will promote an understanding of the genetics and the global movement of M. nubilosa that is severely limiting plantation development.

Original language	English
Pages (from-to)	900-903
Journal	Molecular Ecology Notes
Volume	6
Issue number	3
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01392.x
Publication status	Published - 2006

Keywords

Ascomycete
Forestry
Leaf spot
MLD
Mycosphaerella nubilosa

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10.1111/j.1471-8286.2006.01392.x

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title = "Development of polymorphic microsatellite markers for the Eucalyptus leaf pathogen Mycosphaerella nubilosa",

abstract = "Mycosphaerella nubilosa is one of the most important Eucalyptus leaf pathogens, causing premature defoliation and stunting of growth. The aim of this study was to develop polymorphic microsatellite markers for M. nubilosa. Fifteen primer sets were developed and evaluated for polymorphism. Two primers were monomorphic, three primers did not amplify the desired region and 10 primer pairs were polymorphic. These microsatellite markers will be applied to population biology studies of M. nubilosa collections from several countries. These studies will promote an understanding of the genetics and the global movement of M. nubilosa that is severely limiting plantation development.",

keywords = "Ascomycete, Forestry, Leaf spot, MLD, Mycosphaerella nubilosa",

author = "G.C. Hunter and M.N. Cortinas and B.D. Wingfield and P.W. Crous and M.J. Wingfield",

year = "2006",

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AU - Hunter, G.C.

AU - Cortinas, M.N.

AU - Wingfield, B.D.

AU - Crous, P.W.

AU - Wingfield, M.J.

PY - 2006

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AB - Mycosphaerella nubilosa is one of the most important Eucalyptus leaf pathogens, causing premature defoliation and stunting of growth. The aim of this study was to develop polymorphic microsatellite markers for M. nubilosa. Fifteen primer sets were developed and evaluated for polymorphism. Two primers were monomorphic, three primers did not amplify the desired region and 10 primer pairs were polymorphic. These microsatellite markers will be applied to population biology studies of M. nubilosa collections from several countries. These studies will promote an understanding of the genetics and the global movement of M. nubilosa that is severely limiting plantation development.

KW - Ascomycete

KW - Forestry

KW - Leaf spot

KW - MLD

KW - Mycosphaerella nubilosa

U2 - 10.1111/j.1471-8286.2006.01392.x

DO - 10.1111/j.1471-8286.2006.01392.x

M3 - Article

SN - 1471-8278

VL - 6

SP - 900

EP - 903

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 3

ER -

Development of polymorphic microsatellite markers for the Eucalyptus leaf pathogen Mycosphaerella nubilosa

Abstract

Keywords

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