Development of hypo-allergenic apples: silencing of the major allergen Mal d 1 gene in 'Elstar' apple and the effect of grafting

B.N. Krath, F.D. Eriksen, B.H. Pedersen, L.J.W.J. Gilissen, W.E. van de Weg, L.O. Dragsted

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Many people who are allergic to birch pollen are also allergic to apple fruit, due to cross- allergenicity. Since apples are the most extensively consumed fruit in Europe, it is highly relevant to develop a hypo-allergenic apple. Apples with significantly reduced levels of the allergen, Mal d 1, may allow many apple allergics to eat them without an allergic reaction. We are currently collaborating to develop a hypo-allergenic apple within the European Integrated Research Project, ISAFRUIT (www.isafruit.org). Hypo-allergenic apple plants (Malus × domestica Borkh., ‘Elstar’) with decreased levels of Mal d 1 mRNA were produced by RNA interference (RNAi) technology. Ten genetically modified (GM) apple lines were selected. In vitro plantlets were first transferred to a greenhouse, then grafted onto wild-type M.9 rootstock to promote the development of fruit-producing trees. Levels of Mal d 1 gene silencing were measured repeatedly by quantitative real-time PCR. Compared to leaf samples from wild-type ‘Elstar’, two GM lines showed modest levels of gene silencing (up to 250-fold), whereas the other eight GM lines were significantly silenced (up to10,000-fold) in Mal d 1 gene expression. These levels of silencing were unaffected by grafting, and have been stable over more than 3 years, and throughout all developmental stages.
    Original languageEnglish
    Pages (from-to)52-57
    JournalJournal of Horticultural Science and Biotechnology
    Volume84
    Issue number6 Isafruit Suppl
    Publication statusPublished - 2009

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    Keywords

    • controlled food challenge
    • birch pollen allergen
    • malus-domestica
    • double-blind
    • expression
    • protein
    • cloning
    • bet-v-1
    • fruit

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