Development of FRET biosensors for mammalian and plant systems

D.S. Hamers, L. van Voorst Vader, J.W. Borst, J. Goedhart

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Genetically encoded biosensors are increasingly used in visualising signalling processes in different organisms. Sensors based on green fluorescent protein technology are providing a great opportunity for using Förster resonance energy transfer (FRET) as a tool that allows for monitoring dynamic processes in living cells. The development of these FRET biosensors requires careful selection of fluorophores, substrates and recognition domains. In this review, we will discuss recent developments, strategies to create and optimise FRET biosensors and applications of FRET-based biosensors for use in the two major eukaryotic kingdoms and elaborate on different methods for FRET detection.
Original languageEnglish
Pages (from-to)333-347
JournalProtoplasma
Volume251
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • yellow fluorescent proteins
  • photoactivated localization microscopy
  • resonance energy-transfer
  • living cells
  • mass-spectrometry
  • dynamic-range
  • time
  • ca2+
  • resolution
  • indicators

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