Development of DNA metabarcoding tools for soil fauna

Research output: Contribution to conferenceAbstractAcademic

Abstract

Characterization of the huge soil faunal diversity still relies heavily on the slow and expert-dependent morphological identification. This hampers our ecological understanding of spatial and temporal diversity of many faunal groups, as screening many samples at high taxonomic detail is not a realistic proposition. DNA-based approaches, such as high-throughput DNA metabarcoding, potentially solve this issue. Within the EU FP7 project EcoFINDERS, a large effort has been spent to develop such tools.. Here, I will show results of direct comparisons between different strategies: conventional morphological identification, as well as DNA metabarcoding based on either animals that were removed from the soil or direct soil DNA extracts. Furthermore, I will show some examples of how these tools can be applied to advance our ecological understanding of soil food webs and dispersal patterns.

Conference

ConferenceSymposium de l'Académie d'Agriculture de France & de la Commission Européenne
CountryFrance
CityParijs
Period28/11/1628/11/16

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soil fauna
DNA
soil
food web
animal

Cite this

de Groot, G. A. (2016). Development of DNA metabarcoding tools for soil fauna. 9-9. Abstract from Symposium de l'Académie d'Agriculture de France & de la Commission Européenne, Parijs, France.
de Groot, G.A. / Development of DNA metabarcoding tools for soil fauna. Abstract from Symposium de l'Académie d'Agriculture de France & de la Commission Européenne, Parijs, France.
@conference{99a84f6114ca45c4b4ecf1543845ae57,
title = "Development of DNA metabarcoding tools for soil fauna",
abstract = "Characterization of the huge soil faunal diversity still relies heavily on the slow and expert-dependent morphological identification. This hampers our ecological understanding of spatial and temporal diversity of many faunal groups, as screening many samples at high taxonomic detail is not a realistic proposition. DNA-based approaches, such as high-throughput DNA metabarcoding, potentially solve this issue. Within the EU FP7 project EcoFINDERS, a large effort has been spent to develop such tools.. Here, I will show results of direct comparisons between different strategies: conventional morphological identification, as well as DNA metabarcoding based on either animals that were removed from the soil or direct soil DNA extracts. Furthermore, I will show some examples of how these tools can be applied to advance our ecological understanding of soil food webs and dispersal patterns.",
author = "{de Groot}, G.A.",
year = "2016",
language = "English",
pages = "9--9",
note = "Symposium de l'Acad{\'e}mie d'Agriculture de France & de la Commission Europ{\'e}enne : Lancement en France de 'Global Soil Biodiversity Atlas' ; Conference date: 28-11-2016 Through 28-11-2016",

}

de Groot, GA 2016, 'Development of DNA metabarcoding tools for soil fauna' Symposium de l'Académie d'Agriculture de France & de la Commission Européenne, Parijs, France, 28/11/16 - 28/11/16, pp. 9-9.

Development of DNA metabarcoding tools for soil fauna. / de Groot, G.A.

2016. 9-9 Abstract from Symposium de l'Académie d'Agriculture de France & de la Commission Européenne, Parijs, France.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Development of DNA metabarcoding tools for soil fauna

AU - de Groot, G.A.

PY - 2016

Y1 - 2016

N2 - Characterization of the huge soil faunal diversity still relies heavily on the slow and expert-dependent morphological identification. This hampers our ecological understanding of spatial and temporal diversity of many faunal groups, as screening many samples at high taxonomic detail is not a realistic proposition. DNA-based approaches, such as high-throughput DNA metabarcoding, potentially solve this issue. Within the EU FP7 project EcoFINDERS, a large effort has been spent to develop such tools.. Here, I will show results of direct comparisons between different strategies: conventional morphological identification, as well as DNA metabarcoding based on either animals that were removed from the soil or direct soil DNA extracts. Furthermore, I will show some examples of how these tools can be applied to advance our ecological understanding of soil food webs and dispersal patterns.

AB - Characterization of the huge soil faunal diversity still relies heavily on the slow and expert-dependent morphological identification. This hampers our ecological understanding of spatial and temporal diversity of many faunal groups, as screening many samples at high taxonomic detail is not a realistic proposition. DNA-based approaches, such as high-throughput DNA metabarcoding, potentially solve this issue. Within the EU FP7 project EcoFINDERS, a large effort has been spent to develop such tools.. Here, I will show results of direct comparisons between different strategies: conventional morphological identification, as well as DNA metabarcoding based on either animals that were removed from the soil or direct soil DNA extracts. Furthermore, I will show some examples of how these tools can be applied to advance our ecological understanding of soil food webs and dispersal patterns.

M3 - Abstract

SP - 9

EP - 9

ER -

de Groot GA. Development of DNA metabarcoding tools for soil fauna. 2016. Abstract from Symposium de l'Académie d'Agriculture de France & de la Commission Européenne, Parijs, France.