Development of cisgenic apples with durable resistance to apple scab

S.G. Joshi, J.M. Soriano Soriano, A.J. Kortstee, J.G. Schaart, F.A. Krens, E. Jacobsen, H.J. Schouten

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Most of the apple (Malus × domestica) growers are facing serious disease problems with apple scab which is caused by the fungus Venturia inaequalis. Developing a resistant variety in apple through classical breeding is very slow and inefficient. So, we aim at improving existing apple varieties through a new concept called “cisgenesis” which saves time and effort compared to classical breeding. Malus floribunda proved to be a good source of natural scab resistance genes. The genes HcrVf1 and HcrVf2, consisting of promoter, coding and terminator sequences in their natural configuration, were isolated from Malus floribunda and cloned into the binary vector pMF1. Apple cv. ‘Gala’ was transformed with pMF1 containing HcrVf1 and HcrVf2, individually or in combination. pMF1 can be used to obtain marker-free plants by recombinase-based excision of a fragment carrying undesired gene sequences, such as antibiotic-selection marker genes, leaving behind only the gene(s)-of-interest and one recombination site. Using this vector it is therefore possible to stack several genes by retransformation using the same selection procedure. In order to obtain durable resistance, we have the intention to combine different resistance genes from Malus either by stacking them one by one or by introducing them all together in one T-DNA. Performance of all different types of cisgenic plants will be evaluated by monitoring scab resistance levels phenotypically and by determining gene expression profiles through quantitative RT-PCR
Original languageEnglish
Pages (from-to)403-406
JournalActa Horticulturae
Volume839
DOIs
Publication statusPublished - 2009

Fingerprint

Venturia inaequalis
apples
Malus floribunda
genes
terminator regions
Malus
breeding
new variety
Malus domestica
growers
reverse transcriptase polymerase chain reaction
antibiotics
promoter regions
nucleotide sequences
gene expression
fungi
genetic markers
monitoring
DNA

Cite this

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title = "Development of cisgenic apples with durable resistance to apple scab",
abstract = "Most of the apple (Malus × domestica) growers are facing serious disease problems with apple scab which is caused by the fungus Venturia inaequalis. Developing a resistant variety in apple through classical breeding is very slow and inefficient. So, we aim at improving existing apple varieties through a new concept called “cisgenesis” which saves time and effort compared to classical breeding. Malus floribunda proved to be a good source of natural scab resistance genes. The genes HcrVf1 and HcrVf2, consisting of promoter, coding and terminator sequences in their natural configuration, were isolated from Malus floribunda and cloned into the binary vector pMF1. Apple cv. ‘Gala’ was transformed with pMF1 containing HcrVf1 and HcrVf2, individually or in combination. pMF1 can be used to obtain marker-free plants by recombinase-based excision of a fragment carrying undesired gene sequences, such as antibiotic-selection marker genes, leaving behind only the gene(s)-of-interest and one recombination site. Using this vector it is therefore possible to stack several genes by retransformation using the same selection procedure. In order to obtain durable resistance, we have the intention to combine different resistance genes from Malus either by stacking them one by one or by introducing them all together in one T-DNA. Performance of all different types of cisgenic plants will be evaluated by monitoring scab resistance levels phenotypically and by determining gene expression profiles through quantitative RT-PCR",
author = "S.G. Joshi and {Soriano Soriano}, J.M. and A.J. Kortstee and J.G. Schaart and F.A. Krens and E. Jacobsen and H.J. Schouten",
year = "2009",
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Development of cisgenic apples with durable resistance to apple scab. / Joshi, S.G.; Soriano Soriano, J.M.; Kortstee, A.J.; Schaart, J.G.; Krens, F.A.; Jacobsen, E.; Schouten, H.J.

In: Acta Horticulturae, Vol. 839, 2009, p. 403-406.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Joshi, S.G.

AU - Soriano Soriano, J.M.

AU - Kortstee, A.J.

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AU - Schouten, H.J.

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