Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products

S.M.F. Jeurissen; F.W. Claassen; J. Havlik; E. Bouwmans; N.H.P. Cnubben; E.J.R. Sudhölter; I.M.C.M. Rietjens; T.A. van Beek

doi:10.1016/j.chroma.2006.12.007

Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products

S.M.F. Jeurissen
, F.W. Claassen
, J. Havlik
, E. Bouwmans
, N.H.P. Cnubben
, E.J.R. Sudhölter
, I.M.C.M. Rietjens
, T.A. van Beek

Research output: Contribution to journal › Article › Academic › peer-review

28 Citations (Scopus)

Abstract

An on-line HPLC screening method for detection of inhibitors of human cytochrome P450 1A2 in extracts was developed. HPLC separation of extracts is connected to a continuous methoxyresorufin-O-demethylation (MROD) assay in which recombinant human P450 1A2 converts methoxyresorufin to its fluorescent metabolite resorufin. The system was tested with three P450 1A2 inhibitors, for which minimum detectable amounts (MDA) ranging from 0.7 to 9.5 ng were obtained. Analysis of a kava kava and a basil extract showed that the on-line system is applicable to complex mixtures, since in both extracts, peaks with P450 1A2 inhibiting activity were observed

Original language	English
Pages (from-to)	81-89
Journal	Journal of Chromatography. A, Including electrophoresis and other separation methods
Volume	1141
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2006.12.007
Publication status	Published - 2007

Keywords

tandem mass-spectrometry
biochemical detection
catalytic activity
coupled online
enzymes
methyleugenol
metabolism
bioactivation
fluorescence
activation

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10.1016/j.chroma.2006.12.007

Cite this

Jeurissen, S. M. F., Claassen, F. W., Havlik, J., Bouwmans, E., Cnubben, N. H. P., Sudhölter, E. J. R., Rietjens, I. M. C. M., & van Beek, T. A. (2007). Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products. Journal of Chromatography. A, Including electrophoresis and other separation methods, 1141(1), 81-89. https://doi.org/10.1016/j.chroma.2006.12.007

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title = "Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products",

abstract = "An on-line HPLC screening method for detection of inhibitors of human cytochrome P450 1A2 in extracts was developed. HPLC separation of extracts is connected to a continuous methoxyresorufin-O-demethylation (MROD) assay in which recombinant human P450 1A2 converts methoxyresorufin to its fluorescent metabolite resorufin. The system was tested with three P450 1A2 inhibitors, for which minimum detectable amounts (MDA) ranging from 0.7 to 9.5 ng were obtained. Analysis of a kava kava and a basil extract showed that the on-line system is applicable to complex mixtures, since in both extracts, peaks with P450 1A2 inhibiting activity were observed",

keywords = "tandem mass-spectrometry, biochemical detection, catalytic activity, coupled online, enzymes, methyleugenol, metabolism, bioactivation, fluorescence, activation",

author = "S.M.F. Jeurissen and F.W. Claassen and J. Havlik and E. Bouwmans and N.H.P. Cnubben and E.J.R. Sudh{\"o}lter and I.M.C.M. Rietjens and \{van Beek\}, T.A.",

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Jeurissen, SMF, Claassen, FW, Havlik, J, Bouwmans, E, Cnubben, NHP, Sudhölter, EJR, Rietjens, IMCM & van Beek, TA 2007, 'Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products', Journal of Chromatography. A, Including electrophoresis and other separation methods, vol. 1141, no. 1, pp. 81-89. https://doi.org/10.1016/j.chroma.2006.12.007

Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products. / Jeurissen, S.M.F.; Claassen, F.W.; Havlik, J. et al.
In: Journal of Chromatography. A, Including electrophoresis and other separation methods, Vol. 1141, No. 1, 2007, p. 81-89.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products

AU - Jeurissen, S.M.F.

AU - Claassen, F.W.

AU - Havlik, J.

AU - Bouwmans, E.

AU - Cnubben, N.H.P.

AU - Sudhölter, E.J.R.

AU - Rietjens, I.M.C.M.

AU - van Beek, T.A.

PY - 2007

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N2 - An on-line HPLC screening method for detection of inhibitors of human cytochrome P450 1A2 in extracts was developed. HPLC separation of extracts is connected to a continuous methoxyresorufin-O-demethylation (MROD) assay in which recombinant human P450 1A2 converts methoxyresorufin to its fluorescent metabolite resorufin. The system was tested with three P450 1A2 inhibitors, for which minimum detectable amounts (MDA) ranging from 0.7 to 9.5 ng were obtained. Analysis of a kava kava and a basil extract showed that the on-line system is applicable to complex mixtures, since in both extracts, peaks with P450 1A2 inhibiting activity were observed

AB - An on-line HPLC screening method for detection of inhibitors of human cytochrome P450 1A2 in extracts was developed. HPLC separation of extracts is connected to a continuous methoxyresorufin-O-demethylation (MROD) assay in which recombinant human P450 1A2 converts methoxyresorufin to its fluorescent metabolite resorufin. The system was tested with three P450 1A2 inhibitors, for which minimum detectable amounts (MDA) ranging from 0.7 to 9.5 ng were obtained. Analysis of a kava kava and a basil extract showed that the on-line system is applicable to complex mixtures, since in both extracts, peaks with P450 1A2 inhibiting activity were observed

KW - tandem mass-spectrometry

KW - biochemical detection

KW - catalytic activity

KW - coupled online

KW - enzymes

KW - methyleugenol

KW - metabolism

KW - bioactivation

KW - fluorescence

KW - activation

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DO - 10.1016/j.chroma.2006.12.007

M3 - Article

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VL - 1141

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JO - Journal of Chromatography. A, Including electrophoresis and other separation methods

JF - Journal of Chromatography. A, Including electrophoresis and other separation methods

IS - 1

ER -

Jeurissen SMF, Claassen FW, Havlik J, Bouwmans E, Cnubben NHP, Sudhölter EJR et al. Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products. Journal of Chromatography. A, Including electrophoresis and other separation methods. 2007;1141(1):81-89. doi: 10.1016/j.chroma.2006.12.007

Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products

Abstract

Keywords

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