Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles

A.P. Walczak

Research output: Thesisinternal PhD, WUAcademic

Abstract

Title of the PhD thesis: Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles

The number of food-related products containing nanoparticles (NPs) increases. To understand the safety of such products, the potential uptake of these NPs following consumption needs to be assessed. In normal safety assessment studies this is investigated using animal models. For scientific, ethical and economical reasons, there is a demand to refine, reduce and replace animal testing by developing in vitro alternatives for hazard characterization. In this thesis an in vitro model for the prediction of the uptake of NPs in the human body after consumption was developed. The model consists of two parts. The first part is a laboratory incubation model mimicking human digestion in mouth, stomach and intestine. For the second part, human intestinal wall cells are used to assess the uptake of nanoparticles. The two models were combined into the integrated in vitro model to take into consideration the potential effect of digestion on nanoparticle uptake in the gut. The main outcome of the work is that the cell-based integrated in vitro model can be used to evaluate which NPs are likely taken up by the body at the highest rate. The size of NPs and the type of chemical groups on their surface greatly influenced the uptake of NPs. The developed model can be used to prioritize the NPs for additional investigations. Using this model in the safety assessment of NPs would reduce the number of animals used in safety assessment.

 

LanguageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Rietjens, Ivonne, Promotor
  • Bouwmeester, Hans, Co-promotor
  • Hendriksen, Peter, Co-promotor
Award date5 Jan 2015
Place of PublicationWageningen
Publisher
Print ISBNs9789462572201
Publication statusPublished - 2015

Fingerprint

Nanoparticles
Biological Availability
Safety
Digestion
In Vitro Techniques
Human Body
Cell Wall
Intestines
Mouth
Stomach
Animal Models
Food

Keywords

  • nanotechnology
  • particles
  • in vitro
  • ingestion
  • bioavailability
  • human nutrition research
  • risk assessment

Cite this

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title = "Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles",
abstract = "Title of the PhD thesis: Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles The number of food-related products containing nanoparticles (NPs) increases. To understand the safety of such products, the potential uptake of these NPs following consumption needs to be assessed. In normal safety assessment studies this is investigated using animal models. For scientific, ethical and economical reasons, there is a demand to refine, reduce and replace animal testing by developing in vitro alternatives for hazard characterization. In this thesis an in vitro model for the prediction of the uptake of NPs in the human body after consumption was developed. The model consists of two parts. The first part is a laboratory incubation model mimicking human digestion in mouth, stomach and intestine. For the second part, human intestinal wall cells are used to assess the uptake of nanoparticles. The two models were combined into the integrated in vitro model to take into consideration the potential effect of digestion on nanoparticle uptake in the gut. The main outcome of the work is that the cell-based integrated in vitro model can be used to evaluate which NPs are likely taken up by the body at the highest rate. The size of NPs and the type of chemical groups on their surface greatly influenced the uptake of NPs. The developed model can be used to prioritize the NPs for additional investigations. Using this model in the safety assessment of NPs would reduce the number of animals used in safety assessment.  ",
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author = "A.P. Walczak",
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Walczak, AP 2015, 'Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles', Doctor of Philosophy, Wageningen University, Wageningen.

Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles. / Walczak, A.P.

Wageningen : Wageningen University, 2015. 153 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles

AU - Walczak, A.P.

N1 - WU thesis 5950

PY - 2015

Y1 - 2015

N2 - Title of the PhD thesis: Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles The number of food-related products containing nanoparticles (NPs) increases. To understand the safety of such products, the potential uptake of these NPs following consumption needs to be assessed. In normal safety assessment studies this is investigated using animal models. For scientific, ethical and economical reasons, there is a demand to refine, reduce and replace animal testing by developing in vitro alternatives for hazard characterization. In this thesis an in vitro model for the prediction of the uptake of NPs in the human body after consumption was developed. The model consists of two parts. The first part is a laboratory incubation model mimicking human digestion in mouth, stomach and intestine. For the second part, human intestinal wall cells are used to assess the uptake of nanoparticles. The two models were combined into the integrated in vitro model to take into consideration the potential effect of digestion on nanoparticle uptake in the gut. The main outcome of the work is that the cell-based integrated in vitro model can be used to evaluate which NPs are likely taken up by the body at the highest rate. The size of NPs and the type of chemical groups on their surface greatly influenced the uptake of NPs. The developed model can be used to prioritize the NPs for additional investigations. Using this model in the safety assessment of NPs would reduce the number of animals used in safety assessment.  

AB - Title of the PhD thesis: Development of an integrated in vitro model for the prediction of oral bioavailability of nanoparticles The number of food-related products containing nanoparticles (NPs) increases. To understand the safety of such products, the potential uptake of these NPs following consumption needs to be assessed. In normal safety assessment studies this is investigated using animal models. For scientific, ethical and economical reasons, there is a demand to refine, reduce and replace animal testing by developing in vitro alternatives for hazard characterization. In this thesis an in vitro model for the prediction of the uptake of NPs in the human body after consumption was developed. The model consists of two parts. The first part is a laboratory incubation model mimicking human digestion in mouth, stomach and intestine. For the second part, human intestinal wall cells are used to assess the uptake of nanoparticles. The two models were combined into the integrated in vitro model to take into consideration the potential effect of digestion on nanoparticle uptake in the gut. The main outcome of the work is that the cell-based integrated in vitro model can be used to evaluate which NPs are likely taken up by the body at the highest rate. The size of NPs and the type of chemical groups on their surface greatly influenced the uptake of NPs. The developed model can be used to prioritize the NPs for additional investigations. Using this model in the safety assessment of NPs would reduce the number of animals used in safety assessment.  

KW - nanotechnologie

KW - deeltjes

KW - in vitro

KW - inname

KW - biologische beschikbaarheid

KW - voedingsonderzoek bij de mens

KW - risicoschatting

KW - nanotechnology

KW - particles

KW - in vitro

KW - ingestion

KW - bioavailability

KW - human nutrition research

KW - risk assessment

M3 - internal PhD, WU

SN - 9789462572201

PB - Wageningen University

CY - Wageningen

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