Development of an in vitro protocol to screen Clavibacter michiganensis subsp. michiganensis pathogenicity in different Solanum species.

M.M. Mohd Nadzir, Flavia Vieira Lelis, B. Thapa, Afrida Ali, R.G.F. Visser, A.W. van Heusden, J.M. van der Wolf

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Clavibacter michiganensis subsp. michiganensis (Cmm) is a quarantine organism in Europe and in many other countries. It is one of the most severe bacterial pathogens affecting tomato. Screening tomato plants for their resistance level
to Cmm requires a large amount of space under quarantine conditions and is therefore costly. This project developed a new inoculation protocol on in vitro tomato plants to facilitate a more economic and higher throughput disease screening. A new method using the PathoScreen system was tested to localize green fluorescent protein-tagged Cmm in planta and to quantify the pathogen based on the percentage of corrected GFP (cGFP%). The system was sensitive in detecting the GFP-tagged Cmm in the shoots, but in the roots a high autofluorescence masked detection and thus sensitivity of the assay. The in vitro protocol was tested on several wild relatives of tomato, which were previously screened in a greenhouse assay. The correlation between wilt symptoms in vitro and wilt symptoms in the greenhouse was overall moderate (r = 0.6462). The protocol worked well in differentiating the two parents that were used in the mapping studies. This study shows that the in vitro protocol can be efficiently used for resistance breeding in many tomato genotypes.
LanguageEnglish
Pages42-48
JournalPlant Pathology
Volume68
Issue number1
Early online date3 Aug 2018
DOIs
Publication statusPublished - Jan 2019

Fingerprint

Clavibacter michiganensis subsp. michiganensis
Solanum
Lycopersicon esculentum
Virulence
pathogenicity
tomatoes
Quarantine
quarantine
signs and symptoms (plants)
screening
greenhouses
pathogens
wild relatives
assays
Plantae
Green Fluorescent Proteins
green fluorescent protein
Breeding
Genotype
Economics

Keywords

  • Clavibacter
  • Cmm
  • disease screening
  • in vitro
  • PathoScreen
  • tomato

Cite this

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title = "Development of an in vitro protocol to screen Clavibacter michiganensis subsp. michiganensis pathogenicity in different Solanum species.",
abstract = "Clavibacter michiganensis subsp. michiganensis (Cmm) is a quarantine organism in Europe and in many other countries. It is one of the most severe bacterial pathogens affecting tomato. Screening tomato plants for their resistance levelto Cmm requires a large amount of space under quarantine conditions and is therefore costly. This project developed a new inoculation protocol on in vitro tomato plants to facilitate a more economic and higher throughput disease screening. A new method using the PathoScreen system was tested to localize green fluorescent protein-tagged Cmm in planta and to quantify the pathogen based on the percentage of corrected GFP (cGFP{\%}). The system was sensitive in detecting the GFP-tagged Cmm in the shoots, but in the roots a high autofluorescence masked detection and thus sensitivity of the assay. The in vitro protocol was tested on several wild relatives of tomato, which were previously screened in a greenhouse assay. The correlation between wilt symptoms in vitro and wilt symptoms in the greenhouse was overall moderate (r = 0.6462). The protocol worked well in differentiating the two parents that were used in the mapping studies. This study shows that the in vitro protocol can be efficiently used for resistance breeding in many tomato genotypes.",
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Development of an in vitro protocol to screen Clavibacter michiganensis subsp. michiganensis pathogenicity in different Solanum species. / Mohd Nadzir, M.M.; Vieira Lelis, Flavia; Thapa, B.; Ali, Afrida; Visser, R.G.F.; van Heusden, A.W.; van der Wolf, J.M.

In: Plant Pathology, Vol. 68, No. 1, 01.2019, p. 42-48.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Mohd Nadzir, M.M.

AU - Vieira Lelis, Flavia

AU - Thapa, B.

AU - Ali, Afrida

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