Development of an automated system for isolation and purification of humic substances

A. van Zomeren, E. van der Weij-Zuiver, R.N.J. Comans

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Characterization of humic substances (HS) in environmental samples generally involves labor-intensive and time-consuming isolation and purification procedures. In this paper, the development of an automated system for HS isolation and purification is described. The novelty of the developed system lies in the way the multiple liquids and columns used in the isolation/purification procedure are handled in both forward and back-elution mode by solenoid valves. The automated procedure significantly reduces the total throughput time needed, from 6¿7 days to 48 h, and the amount of labor to obtain purified HS for further characterization. Chemical characterization of purified HS showed that results were in good agreement with previously published values for HS from a variety of sources, including the IHSS standard HS collection. It was also shown that the general properties of HS were consistent among the different source materials (soil, waste, aquatic) used in this study. The developed system greatly facilitates isolation and characterization of HS and reduces the risk of potential (time-dependent) alteration of HS properties in the manual procedure
Original languageEnglish
Pages (from-to)2365-2370
JournalAnalytical and Bioanalytical Chemistry
Volume391
Issue number6
DOIs
Publication statusPublished - 2008

Keywords

  • preparative isolation
  • natural-waters

Fingerprint Dive into the research topics of 'Development of an automated system for isolation and purification of humic substances'. Together they form a unique fingerprint.

  • Cite this