Development of a serological Luminex assay for Trichinella and Salmonella in swine

Research output: Contribution to conferenceAbstractAcademic

Abstract

In order to develop veterinary serological multiplex assays, a singleplex bead-based array on the Luminex platform was developed, and with this experience the study was expanded by building a multiplex serological assay. First a serological Luminex assay was developed for Trichinella in swine. As the developed assay performed comparable to commercial ELISA’s, work on this platform was continued by developing a serological multiplex assay for Salmonella in swine. This assay is based on five LPS variants of the most important serogroups occurring in pigs. The serological multiplex assay for Salmonella performed comparable to a commercial ELISA. The results from this study demonstrate the feasibility of the Luminex platform for multiplex serology. Ultimately, this type of assay can be used for routine screening of porcine serum samples for immune responses against multiple pathogens in one assay.
Original languageEnglish
Number of pages4
Publication statusPublished - 2011
EventProceedings of Safepork 2011, Maastricht, the Netherlands -
Duration: 19 Jun 201222 Jun 2012

Conference

ConferenceProceedings of Safepork 2011, Maastricht, the Netherlands
Period19/06/1222/06/12

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Trichinella
Salmonella
swine
assays
enzyme-linked immunosorbent assay
blood serum
serotypes
immune response
screening
pathogens

Cite this

van der Wal, F. J., Achterberg, R. P., & Maassen, C. B. M. (2011). Development of a serological Luminex assay for Trichinella and Salmonella in swine. Abstract from Proceedings of Safepork 2011, Maastricht, the Netherlands, .
van der Wal, F.J. ; Achterberg, R.P. ; Maassen, C.B.M. / Development of a serological Luminex assay for Trichinella and Salmonella in swine. Abstract from Proceedings of Safepork 2011, Maastricht, the Netherlands, .4 p.
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title = "Development of a serological Luminex assay for Trichinella and Salmonella in swine",
abstract = "In order to develop veterinary serological multiplex assays, a singleplex bead-based array on the Luminex platform was developed, and with this experience the study was expanded by building a multiplex serological assay. First a serological Luminex assay was developed for Trichinella in swine. As the developed assay performed comparable to commercial ELISA’s, work on this platform was continued by developing a serological multiplex assay for Salmonella in swine. This assay is based on five LPS variants of the most important serogroups occurring in pigs. The serological multiplex assay for Salmonella performed comparable to a commercial ELISA. The results from this study demonstrate the feasibility of the Luminex platform for multiplex serology. Ultimately, this type of assay can be used for routine screening of porcine serum samples for immune responses against multiple pathogens in one assay.",
author = "{van der Wal}, F.J. and R.P. Achterberg and C.B.M. Maassen",
year = "2011",
language = "English",
note = "Proceedings of Safepork 2011, Maastricht, the Netherlands ; Conference date: 19-06-2012 Through 22-06-2012",

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van der Wal, FJ, Achterberg, RP & Maassen, CBM 2011, 'Development of a serological Luminex assay for Trichinella and Salmonella in swine' Proceedings of Safepork 2011, Maastricht, the Netherlands, 19/06/12 - 22/06/12, .

Development of a serological Luminex assay for Trichinella and Salmonella in swine. / van der Wal, F.J.; Achterberg, R.P.; Maassen, C.B.M.

2011. Abstract from Proceedings of Safepork 2011, Maastricht, the Netherlands, .

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Development of a serological Luminex assay for Trichinella and Salmonella in swine

AU - van der Wal, F.J.

AU - Achterberg, R.P.

AU - Maassen, C.B.M.

PY - 2011

Y1 - 2011

N2 - In order to develop veterinary serological multiplex assays, a singleplex bead-based array on the Luminex platform was developed, and with this experience the study was expanded by building a multiplex serological assay. First a serological Luminex assay was developed for Trichinella in swine. As the developed assay performed comparable to commercial ELISA’s, work on this platform was continued by developing a serological multiplex assay for Salmonella in swine. This assay is based on five LPS variants of the most important serogroups occurring in pigs. The serological multiplex assay for Salmonella performed comparable to a commercial ELISA. The results from this study demonstrate the feasibility of the Luminex platform for multiplex serology. Ultimately, this type of assay can be used for routine screening of porcine serum samples for immune responses against multiple pathogens in one assay.

AB - In order to develop veterinary serological multiplex assays, a singleplex bead-based array on the Luminex platform was developed, and with this experience the study was expanded by building a multiplex serological assay. First a serological Luminex assay was developed for Trichinella in swine. As the developed assay performed comparable to commercial ELISA’s, work on this platform was continued by developing a serological multiplex assay for Salmonella in swine. This assay is based on five LPS variants of the most important serogroups occurring in pigs. The serological multiplex assay for Salmonella performed comparable to a commercial ELISA. The results from this study demonstrate the feasibility of the Luminex platform for multiplex serology. Ultimately, this type of assay can be used for routine screening of porcine serum samples for immune responses against multiple pathogens in one assay.

M3 - Abstract

ER -

van der Wal FJ, Achterberg RP, Maassen CBM. Development of a serological Luminex assay for Trichinella and Salmonella in swine. 2011. Abstract from Proceedings of Safepork 2011, Maastricht, the Netherlands, .