Development of a new generic extraction method for the analysis of pesticides, mycotoxins, and polycyclic aromatic hydrocarbons in representative animal feed and food samples

Philipp Eyring*, Marc Tienstra, Hans Mol, Susan Strange Herrmann, Peter Have Rasmussen, Henrik Lauritz Frandsen, Mette Erecius Poulsen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Various generic extraction methods have been used to determine pesticide residues, mycotoxins, and polycyclic aromatic hydrocarbons (PAHs) in food and animal feed to ensure consumer safety. However, these methods cannot extract all relevant compounds at an acceptable rate of recovery. This study presents a new extraction method. This new method facilitated the identification of 231 compounds, including 196 pesticides, 11 mycotoxins, and 24 PAHs over a broad range of polarities. These compounds were identified in various sample matrices, including those that are lipid-rich. The processed sample is first extracted with water, acetonitrile, formic acid, and heptane. The addition of ammonium formate results in separation into three phases and enables analysis of the aqueous phase. Solid-phase extraction clean-up procedures were performed as necessary followed by analysis by liquid or gas chromatography and mass spectrometry. Analyte recoveries were typically in the range of 70 – 120% with relative standard deviations below 20%.

Original languageEnglish
Article number129653
JournalFood Chemistry
Volume356
DOIs
Publication statusPublished - 15 Sep 2021

Keywords

  • Aflatoxin B1 (PubChem CID:186907)
  • Benz[a]pyrene (PubChem CID:2336)
  • Chlormequat (PubChem CID:13837)
  • Diquat (PubChem CID:6795)
  • Fenthion (PubChem CID:3346)
  • Fumonisin B1 (PubChem CID:2733487)
  • Gas chromatography-mass spectrometry (GC-MS)
  • Generic extraction method
  • Heptachlor PubChem CID:3589)
  • Liquid chromatography-mass spectrometry (LC-MS)
  • Mycotoxins
  • p,p'-DDT (PubChem CID:3036)
  • Pesticides
  • Polycyclic aromatic hydrocarbons (PAHs)

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