Development of a Nasonia vitripennis outbred laboratory population for genetic analysis

L. van de Zande, S. Ferber, A. de Haan, L.W. Beukeboom, J. van Heerwaarden, B.A. Pannebakker

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne ) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system
Original languageEnglish
Pages (from-to)578-587
JournalMolecular Ecology Resources
Volume14
Issue number3
DOIs
Publication statusPublished - 2014

Keywords

  • parasitoid wasp nasonia
  • local mate competition
  • sex-ratio
  • drosophila-melanogaster
  • natural-populations
  • ectoparasitic wasp
  • hymenoptera
  • genome
  • evolution
  • recombination

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