Development of a Nasonia vitripennis outbred laboratory population for genetic analysis

L. van de Zande, S. Ferber, A. de Haan, L.W. Beukeboom, J. van Heerwaarden, B.A. Pannebakker

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne ) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system
Original languageEnglish
Pages (from-to)578-587
JournalMolecular Ecology Resources
Volume14
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Nasonia
Nasonia vitripennis
Population Genetics
genetic analysis
genetic techniques and protocols
population genetics
Genetic Models
Genome
genome
Population
genetic variation
Developmental Biology
Wasps
Population Density
developmental biology
Biological Sciences
Netherlands
Microsatellite Repeats
Single Nucleotide Polymorphism
effective population size

Keywords

  • parasitoid wasp nasonia
  • local mate competition
  • sex-ratio
  • drosophila-melanogaster
  • natural-populations
  • ectoparasitic wasp
  • hymenoptera
  • genome
  • evolution
  • recombination

Cite this

van de Zande, L. ; Ferber, S. ; de Haan, A. ; Beukeboom, L.W. ; van Heerwaarden, J. ; Pannebakker, B.A. / Development of a Nasonia vitripennis outbred laboratory population for genetic analysis. In: Molecular Ecology Resources. 2014 ; Vol. 14, No. 3. pp. 578-587.
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abstract = "The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne ) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system",
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Development of a Nasonia vitripennis outbred laboratory population for genetic analysis. / van de Zande, L.; Ferber, S.; de Haan, A.; Beukeboom, L.W.; van Heerwaarden, J.; Pannebakker, B.A.

In: Molecular Ecology Resources, Vol. 14, No. 3, 2014, p. 578-587.

Research output: Contribution to journalArticleAcademicpeer-review

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