Development of a molecular assay for the detection of Cucumber mosaic virus and the discrimination of its subgroups I and II

N. Bald-Blume, J.H.W. Bergervoet, E. Maiss*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups. Sequences from GenBank were used to design primers. Additionally, a subgroup specific ELISA was conducted for comparison. This work is part of a project which aims to develop a test for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.
Original languageEnglish
Pages (from-to)35-43
JournalJournal of Virological Methods
Volume243
DOIs
Publication statusPublished - 2017

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Cucumovirus
Technology
Plant Viruses
Nucleic Acid Databases
Capsid Proteins
Nucleic Acids
Virulence
Enzyme-Linked Immunosorbent Assay
Genes

Keywords

  • Cucumber mosaic virus
  • ELISA
  • Luminex xTAG
  • Molecular diagnostics

Cite this

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title = "Development of a molecular assay for the detection of Cucumber mosaic virus and the discrimination of its subgroups I and II",
abstract = "A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups. Sequences from GenBank were used to design primers. Additionally, a subgroup specific ELISA was conducted for comparison. This work is part of a project which aims to develop a test for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.",
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Development of a molecular assay for the detection of Cucumber mosaic virus and the discrimination of its subgroups I and II. / Bald-Blume, N.; Bergervoet, J.H.W.; Maiss, E.

In: Journal of Virological Methods, Vol. 243, 2017, p. 35-43.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Development of a molecular assay for the detection of Cucumber mosaic virus and the discrimination of its subgroups I and II

AU - Bald-Blume, N.

AU - Bergervoet, J.H.W.

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PY - 2017

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N2 - A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups. Sequences from GenBank were used to design primers. Additionally, a subgroup specific ELISA was conducted for comparison. This work is part of a project which aims to develop a test for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.

AB - A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups. Sequences from GenBank were used to design primers. Additionally, a subgroup specific ELISA was conducted for comparison. This work is part of a project which aims to develop a test for the simultaneous detection of various plant pathogens (viral, bacterial and fungal) in plant material.

KW - Cucumber mosaic virus

KW - ELISA

KW - Luminex xTAG

KW - Molecular diagnostics

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VL - 243

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

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