Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria

M. Batchelor, K.L. Hopkins, E. Liebana, P. Slickers, R. Ehricht, M. Mafura, F. Aerestrup, D.J. Mevius, F.A. Clifton-Hadley, M. Woodward, R. Davies, J. Threlfall, F.M. Anjum

    Research output: Contribution to journalArticleAcademicpeer-review

    98 Citations (Scopus)

    Abstract

    We describe the development of a miniaturised microarray for the detection of antimicrobial resistance genes in Gram-negative bacteria. Included on the array are genes encoding resistance to aminoglycosides, trimethoprim, sulphonamides, tetracyclines and ß-lactams, including extended-spectrum ß-lactamases. Validation of the array with control strains demonstrated a 99% correlation between polymerase chain reaction and array results. There was also good correlation between phenotypic and genotypic results for a large panel of Escherichia coli and Salmonella isolates. Some differences were also seen in the number and type of resistance genes harboured by E. coli and Salmonella strains. The array provides an effective, fast and simple method for detection of resistance genes in clinical isolates suitable for use in diagnostic laboratories, which in future will help to understand the epidemiology of isolates and to detect gene linkage in bacterial populations.
    Original languageEnglish
    Pages (from-to)440-451
    JournalInternational Journal of Antimicrobial Agents
    Volume31
    Issue number5
    DOIs
    Publication statusPublished - 2008

    Keywords

    • escherichia-coli
    • beta-lactamases
    • staphylococcus-aureus
    • dna microarrays
    • humans
    • animals
    • england
    • wales

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