Development of a high-throughput microsatellite typing approach for forensic and population genetic analysis of wild and domestic African Bovini

B.J. Greyling, P. Kryger, S. du Plessis, W.F. van Hooft, P. van Helden, W.M. Getz, A.D.S. Bastos

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Conservation management and forensic traceability of African buffalo and cattle rely on the timely provision of unbiased and accurate genetic information. An approach in which 17 cattle microsatellite markers are co-electrophoresed, following amplification in three core multiplex reactions was established for this purpose. Mean allelic richness per locus was 8.24 and 6.47, for buffalo and Bonsmara cattle, respectively, whilst an unbiased match probability of 6.5x×10-17 and 1.03 × 10-16 was obtained for each. These results confirm the usefulness of this rapid, cost-effective typing approach for forensic, paternity and fine-scale genetic analyses of wild and domestic African Bovini tribe members
Original languageEnglish
Pages (from-to)655-660
JournalAfrican journal of biotechnology
Volume7
Issue number5
Publication statusPublished - 2008

Keywords

  • syncerus caffer
  • polymorphic bovine
  • southern africa
  • linkage map
  • buffalo
  • markers
  • identification
  • cattle

Fingerprint Dive into the research topics of 'Development of a high-throughput microsatellite typing approach for forensic and population genetic analysis of wild and domestic African Bovini'. Together they form a unique fingerprint.

Cite this