Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum

H.P. Li, A.B. Wu, C.S. Zhao, O.E. Scholten, H.J.M. Löffler, Y.C. Liao

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    50 Citations (Scopus)

    Abstract

    Based on the intergenic sequences of Tri5¿Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of Fusarium graminearum. Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 F. graminearum isolates from 12 provinces of China showed that 310 were DON-chemotypes and 54 were NIV-chemotypes. Sequence analyses revealed that DON-chemotypes display more variations than NIV-chemotypes. This PCR assay could be used to detect mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody-based mycotoxin tests
    Original languageEnglish
    Pages (from-to)505-511
    JournalFEMS Microbiology Letters
    Volume243
    Issue number2
    DOIs
    Publication statusPublished - 2005

    Keywords

    • gibberella-zeae
    • trichothecene biosynthesis
    • natural occurrence
    • mycotoxins
    • wheat
    • identification
    • sporotrichioides
    • quantification
    • chromatography
    • polymorphism

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