Abstract
Based on the intergenic sequences of Tri5¿Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of Fusarium graminearum. Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 F. graminearum isolates from 12 provinces of China showed that 310 were DON-chemotypes and 54 were NIV-chemotypes. Sequence analyses revealed that DON-chemotypes display more variations than NIV-chemotypes. This PCR assay could be used to detect mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody-based mycotoxin tests
Original language | English |
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Pages (from-to) | 505-511 |
Journal | FEMS Microbiology Letters |
Volume | 243 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2005 |
Keywords
- gibberella-zeae
- trichothecene biosynthesis
- natural occurrence
- mycotoxins
- wheat
- identification
- sporotrichioides
- quantification
- chromatography
- polymorphism