Development of a general potexvirus cDNA and PCR primer set

R.A.A. van der Vlugt, M. Berendsen

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)

Abstract

A method was developed for the detection of viruses from the genus Potexvirus. Following alignment of full-length RNA sequences and deduced amino acid sequences of 10 different viruses from the genus Potexvirus, a number of conserved sequence motifs were identified in the viral replicase-encoding region. Seven different primers based on these motifs were tested for their efficiency as potexvirus group-specific cDNA and/or PCR primer. Several combinations of primers proved capable of generating DNA fragments for each of six different potexviruses tested. One cDNA primer in combination with one PCR primers set proved most successful in reliably generating discrete PCR products. Application of this set to a number of different potexviruses, some for which no sequence data had been published yet, resulted in amplification of virus-specific PCR products for all viruses tested. Sequence analysis of cloned PCR products confirmed their identity. This general potexvirus primer set can be useful for the identification of (unknown) potexvirus infections
Original languageEnglish
Pages (from-to)367-371
JournalEuropean Journal of Plant Pathology
Volume108
Issue number4
DOIs
Publication statusPublished - 2002

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