Development of a biocatalytic process for the production of c6-aldehydes from vegetable oils by soybean lipoxygenase and recombinant hydroperoxide lyase

M.A. Noordermeer, W. van der Goot, A.J. van Kooij, J.W. Veldsink, G.A. Veldink, J.F.G. Vliegenthart

    Research output: Contribution to journalArticleAcademicpeer-review

    49 Citations (Scopus)

    Abstract

    Volatile C6- and C9-aldehydes and alcohols are widely used as food flavors to reconstitute the "fresh green" odor of fruits and vegetables lost during processing. To meet the high demand for natural flavors, an efficient, cheap, and versatile biocatalytic process was developed to produce C6-aldehydes on a large scale. Vegetable oils were converted by soybean lipoxygenase and recombinant hydroperoxide lyase into hexanal and (2E)- or (3Z)-hexenal. In contrast to plant extracts, generally used as enzyme sources, high molar conversions were obtained with recombinant hydroperoxide lyase (50␏or hexanal and 26␏or hexenal formation), and no side products were formed. Furthermore, recombinant hydroperoxide lyase lacks isomerase activity, allowing production of (3Z)-hexenal, which could not be obtained in previously described processes. Recombinant hydroperoxide lyase is stable and can be stored at 4 C for 1 month without significant loss of activity.
    Original languageEnglish
    Pages (from-to)4270-4274
    JournalJournal of Agricultural and Food Chemistry
    Volume50
    Issue number15
    DOIs
    Publication statusPublished - 2002

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