Development and validation of real-time PCR screening methods for detection of cry1a.105 and cry2ab2 genes in genetically modified organisms

A.Z. Dinon, T.W. Prins, J.P. van Dijk, C.M. Arisi, I.M.J. Scholtens-Toma, E.J. Kok

    Research output: Contribution to journalArticleAcademicpeer-review

    38 Citations (Scopus)

    Abstract

    Primers and probes were developed for the element-specific detection of cry1A.105 and cry2Ab2 genes, based on their DNA sequence as present in GM maize MON89034. Cry genes are present in many genetically modified (GM) plants and they are important targets for developing GMO element-specific detection methods. Element-specific methods can be of use to screen for the presence of GMOs in food and feed supply chains. Moreover, a combination of GMO elements may indicate the potential presence of unapproved GMOs (UGMs). Primer-probe combinations were evaluated in terms of specificity, efficiency and limit of detection. Except for specificity, the complete experiment was performed in 9 PCR runs, on 9 different days and by testing 8 DNA concentrations. The results showed a high specificity and efficiency for cry1A.105 and cry2Ab2 detection. The limit of detection was between 0.05 and 0.01 ng DNA per PCR reaction for both assays. These data confirm the applicability of these new primer-probe combinations for element detection that can contribute to the screening for GM and UGM crops in food and feed samples.
    Original languageEnglish
    Pages (from-to)1433-1442
    JournalAnalytical and Bioanalytical Chemistry
    Volume400
    Issue number5
    DOIs
    Publication statusPublished - 2011

    Keywords

    • bacillus-thuringiensis
    • glyphosate-tolerant
    • crystal proteins
    • reference corn
    • fed diets
    • identification
    • performance
    • gmos

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