Development and validation of in vitro bioassays for thyroid hormone receptor mediated endocrine disruption

J. de Freitas

Research output: Thesisinternal PhD, WUAcademic

Abstract

Thyroid hormones regulate crucial processes in vertebrates such as reproduction, development and energy metabolism. Endocrine disruption via the thyroid hormone system is gaining more attention both from scientists and regulators, because of the increasing incidence of hormone-related cancers and developmental defects, and the requirement that newly marketed compounds are tested for thyroid hormone disruption. To reduce the number of experimental animals used and to increase the insight into the mechanisms of toxic interference with the thyroid hormone receptor function, we developed and validated functional in vitro bioassays for thyroid hormone receptor-mediated toxicity. These assays enable quick identification and quantification of specific thyroid hormone receptor disrupting potency of compounds and contribute to the further establishment of a battery of in vitro tests for hazard identification of thyroid active compounds.

LanguageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Murk, Tinka, Promotor
  • Rietjens, Ivonne, Promotor
  • Furlow, J.D., Co-promotor, External person
Award date17 Dec 2012
Place of Publication[S.l.]
Publisher
Print ISBNs9789461734372
Publication statusPublished - 2012

Fingerprint

Thyroid Hormone Receptors
Thyroid Hormones
Biological Assay
Poisons
Energy Metabolism
Reproduction
Vertebrates
Thyroid Gland
Hormones
Incidence
In Vitro Techniques
Neoplasms

Keywords

  • endocrine disruptors
  • bioassays
  • in vitro
  • thyroid hormones

Cite this

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title = "Development and validation of in vitro bioassays for thyroid hormone receptor mediated endocrine disruption",
abstract = "Thyroid hormones regulate crucial processes in vertebrates such as reproduction, development and energy metabolism. Endocrine disruption via the thyroid hormone system is gaining more attention both from scientists and regulators, because of the increasing incidence of hormone-related cancers and developmental defects, and the requirement that newly marketed compounds are tested for thyroid hormone disruption. To reduce the number of experimental animals used and to increase the insight into the mechanisms of toxic interference with the thyroid hormone receptor function, we developed and validated functional in vitro bioassays for thyroid hormone receptor-mediated toxicity. These assays enable quick identification and quantification of specific thyroid hormone receptor disrupting potency of compounds and contribute to the further establishment of a battery of in vitro tests for hazard identification of thyroid active compounds.",
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de Freitas, J 2012, 'Development and validation of in vitro bioassays for thyroid hormone receptor mediated endocrine disruption', Doctor of Philosophy, Wageningen University, [S.l.].

Development and validation of in vitro bioassays for thyroid hormone receptor mediated endocrine disruption. / de Freitas, J.

[S.l.] : s.n., 2012. 192 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Development and validation of in vitro bioassays for thyroid hormone receptor mediated endocrine disruption

AU - de Freitas, J.

N1 - WU thesis, no. 5378

PY - 2012

Y1 - 2012

N2 - Thyroid hormones regulate crucial processes in vertebrates such as reproduction, development and energy metabolism. Endocrine disruption via the thyroid hormone system is gaining more attention both from scientists and regulators, because of the increasing incidence of hormone-related cancers and developmental defects, and the requirement that newly marketed compounds are tested for thyroid hormone disruption. To reduce the number of experimental animals used and to increase the insight into the mechanisms of toxic interference with the thyroid hormone receptor function, we developed and validated functional in vitro bioassays for thyroid hormone receptor-mediated toxicity. These assays enable quick identification and quantification of specific thyroid hormone receptor disrupting potency of compounds and contribute to the further establishment of a battery of in vitro tests for hazard identification of thyroid active compounds.

AB - Thyroid hormones regulate crucial processes in vertebrates such as reproduction, development and energy metabolism. Endocrine disruption via the thyroid hormone system is gaining more attention both from scientists and regulators, because of the increasing incidence of hormone-related cancers and developmental defects, and the requirement that newly marketed compounds are tested for thyroid hormone disruption. To reduce the number of experimental animals used and to increase the insight into the mechanisms of toxic interference with the thyroid hormone receptor function, we developed and validated functional in vitro bioassays for thyroid hormone receptor-mediated toxicity. These assays enable quick identification and quantification of specific thyroid hormone receptor disrupting potency of compounds and contribute to the further establishment of a battery of in vitro tests for hazard identification of thyroid active compounds.

KW - hormoonverstoorders

KW - biotesten

KW - in vitro

KW - schildklierhormonen

KW - endocrine disruptors

KW - bioassays

KW - in vitro

KW - thyroid hormones

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