Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry

M.G.J. Balvers, K.C.M. Verhoeckx, R.F. Witkamp

Research output: Contribution to journalArticleAcademicpeer-review

65 Citations (Scopus)

Abstract

A sensitive and specific LC¿MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-¿-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 ¿g/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels
Original languageEnglish
Pages (from-to)1583-1590
JournalJournal of Chromatography. B, Analytical technologies in the biomedical and life sciences
Volume877
Issue number14-15
DOIs
Publication statusPublished - 2009

Keywords

  • fatty-acid amides
  • cannabinoid receptor
  • rat-brain
  • endogenous cannabinoids
  • anandamide
  • pain
  • palmitoylethanolamide
  • inflammation
  • disorders
  • system

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