Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry

M.G.J. Balvers, K.C.M. Verhoeckx, R.F. Witkamp

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Abstract

A sensitive and specific LC¿MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-¿-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 ¿g/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels
Original languageEnglish
Pages (from-to)1583-1590
JournalJournal of Chromatography. B, Analytical technologies in the biomedical and life sciences
Volume877
Issue number14-15
DOIs
Publication statusPublished - 2009

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Plasma (human)
Endocannabinoids
Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Dopamine
Glyceryl Ethers
Glycine
Solid Phase Extraction
2-arachidonylglycerol
anandamide
acetonitrile

Keywords

  • fatty-acid amides
  • cannabinoid receptor
  • rat-brain
  • endogenous cannabinoids
  • anandamide
  • pain
  • palmitoylethanolamide
  • inflammation
  • disorders
  • system

Cite this

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title = "Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry",
abstract = "A sensitive and specific LC¿MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-¿-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 ¿g/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80{\%} (except stearoyl ethanolamide at lowest spike level) at all spike levels",
keywords = "fatty-acid amides, cannabinoid receptor, rat-brain, endogenous cannabinoids, anandamide, pain, palmitoylethanolamide, inflammation, disorders, system",
author = "M.G.J. Balvers and K.C.M. Verhoeckx and R.F. Witkamp",
year = "2009",
doi = "10.1016/j.jchromb.2009.04.010",
language = "English",
volume = "877",
pages = "1583--1590",
journal = "Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences",
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AU - Balvers, M.G.J.

AU - Verhoeckx, K.C.M.

AU - Witkamp, R.F.

PY - 2009

Y1 - 2009

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AB - A sensitive and specific LC¿MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-¿-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 ¿g/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels

KW - fatty-acid amides

KW - cannabinoid receptor

KW - rat-brain

KW - endogenous cannabinoids

KW - anandamide

KW - pain

KW - palmitoylethanolamide

KW - inflammation

KW - disorders

KW - system

U2 - 10.1016/j.jchromb.2009.04.010

DO - 10.1016/j.jchromb.2009.04.010

M3 - Article

VL - 877

SP - 1583

EP - 1590

JO - Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences

JF - Journal of Chromatography. B, Analytical technologies in the biomedical and life sciences

SN - 1570-0232

IS - 14-15

ER -