Abstract
A series of highly monodisperse silicon nanoparticles (Si NP) with either
positively (amine), neutral (azide) or negatively (carboxylic acid) charged
covalently attached organic monolayers were synthesized and investigated for
their cytotoxicity. Infrared data confirmed the presence of these covalently
attached surface groups. The Si NP were characterized by absorption and
fluorescence spectroscopy. The cytotoxicity was investigated in Caco -2 cells by
determining the cell viability and proliferation. The EC50 values for the Si NP
ranged from 20 μg/l for the amine-terminated Si NP, via 550–850 μg/l for the
azide-terminated Si NP to non-toxic (no measureable EC50) for the carboxylic acid -
terminated Si NP. These results indicated a trend in cytotoxicity, depending on
surface charge, i.e. that positively charged Si NP we re more cytotoxic than
negatively charged Si NP. Interestingly, it appeared that the cytotoxicity of the
amine terminated Si NP (Si NP-NH2) depended strongly on the presence of fetal calf
serum (FCS) in the medium.
| Language | English |
|---|---|
| Qualification | Doctor of Philosophy |
| Awarding Institution |
|
| Supervisors/Advisors |
|
| Award date | 7 Sep 2012 |
| Place of Publication | S.l. |
| Publisher | |
| Print ISBNs | 9789461733481 |
| Publication status | Published - 2012 |
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Keywords
- nanotechnology
- particles
- health hazards
- risk assessment
- cytotoxicity
- rats
- bioavailability
- mode of action
Cite this
}
Development and hazard assessment of nanoparticles. / Bhattacharjee, S.
S.l. : s.n., 2012. 240 p.Research output: Thesis › internal PhD, WU › Academic
TY - THES
T1 - Development and hazard assessment of nanoparticles
AU - Bhattacharjee, S.
N1 - WU thesis 5286
PY - 2012
Y1 - 2012
N2 - A series of highly monodisperse silicon nanoparticles (Si NP) with either positively (amine), neutral (azide) or negatively (carboxylic acid) charged covalently attached organic monolayers were synthesized and investigated for their cytotoxicity. Infrared data confirmed the presence of these covalently attached surface groups. The Si NP were characterized by absorption and fluorescence spectroscopy. The cytotoxicity was investigated in Caco -2 cells by determining the cell viability and proliferation. The EC50 values for the Si NP ranged from 20 μg/l for the amine-terminated Si NP, via 550–850 μg/l for the azide-terminated Si NP to non-toxic (no measureable EC50) for the carboxylic acid - terminated Si NP. These results indicated a trend in cytotoxicity, depending on surface charge, i.e. that positively charged Si NP we re more cytotoxic than negatively charged Si NP. Interestingly, it appeared that the cytotoxicity of the amine terminated Si NP (Si NP-NH2) depended strongly on the presence of fetal calf serum (FCS) in the medium.
AB - A series of highly monodisperse silicon nanoparticles (Si NP) with either positively (amine), neutral (azide) or negatively (carboxylic acid) charged covalently attached organic monolayers were synthesized and investigated for their cytotoxicity. Infrared data confirmed the presence of these covalently attached surface groups. The Si NP were characterized by absorption and fluorescence spectroscopy. The cytotoxicity was investigated in Caco -2 cells by determining the cell viability and proliferation. The EC50 values for the Si NP ranged from 20 μg/l for the amine-terminated Si NP, via 550–850 μg/l for the azide-terminated Si NP to non-toxic (no measureable EC50) for the carboxylic acid - terminated Si NP. These results indicated a trend in cytotoxicity, depending on surface charge, i.e. that positively charged Si NP we re more cytotoxic than negatively charged Si NP. Interestingly, it appeared that the cytotoxicity of the amine terminated Si NP (Si NP-NH2) depended strongly on the presence of fetal calf serum (FCS) in the medium.
KW - nanotechnologie
KW - deeltjes
KW - gezondheidsgevaren
KW - risicoschatting
KW - cytotoxiciteit
KW - ratten
KW - biologische beschikbaarheid
KW - werkwijze
KW - nanotechnology
KW - particles
KW - health hazards
KW - risk assessment
KW - cytotoxicity
KW - rats
KW - bioavailability
KW - mode of action
M3 - internal PhD, WU
SN - 9789461733481
PB - s.n.
CY - S.l.
ER -