Development and hazard assessment of nanoparticles

S. Bhattacharjee

Research output: Thesisinternal PhD, WUAcademic

Abstract

A series of highly monodisperse silicon nanoparticles (Si NP) with either
positively (amine), neutral (azide) or negatively (carboxylic acid) charged
covalently attached organic monolayers were synthesized and investigated for
their cytotoxicity. Infrared data confirmed the presence of these covalently
attached surface groups. The Si NP were characterized by absorption and
fluorescence spectroscopy. The cytotoxicity was investigated in Caco -2 cells by
determining the cell viability and proliferation. The EC50 values for the Si NP
ranged from 20 μg/l for the amine-terminated Si NP, via 550–850 μg/l for the
azide-terminated Si NP to non-toxic (no measureable EC50) for the carboxylic acid -
terminated Si NP. These results indicated a trend in cytotoxicity, depending on
surface charge, i.e. that positively charged Si NP we re more cytotoxic than
negatively charged Si NP. Interestingly, it appeared that the cytotoxicity of the
amine terminated Si NP (Si NP-NH2) depended strongly on the presence of fetal calf
serum (FCS) in the medium.
 

LanguageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Rietjens, Ivonne, Promotor
  • Zuilhof, Han, Promotor
  • Alink, Gerrit, Co-promotor
  • Marcelis, Ton, Co-promotor
Award date7 Sep 2012
Place of PublicationS.l.
Publisher
Print ISBNs9789461733481
Publication statusPublished - 2012

Fingerprint

Silicon
Hazards
Nanoparticles
Cytotoxicity
Amines
Azides
Carboxylic Acids
Fluorescence spectroscopy
Surface charge
Absorption spectroscopy
Monolayers
Cells
Infrared radiation

Keywords

  • nanotechnology
  • particles
  • health hazards
  • risk assessment
  • cytotoxicity
  • rats
  • bioavailability
  • mode of action

Cite this

Bhattacharjee, S. (2012). Development and hazard assessment of nanoparticles. S.l.: s.n.
Bhattacharjee, S.. / Development and hazard assessment of nanoparticles. S.l. : s.n., 2012. 240 p.
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abstract = "A series of highly monodisperse silicon nanoparticles (Si NP) with either positively (amine), neutral (azide) or negatively (carboxylic acid) charged covalently attached organic monolayers were synthesized and investigated for their cytotoxicity. Infrared data confirmed the presence of these covalently attached surface groups. The Si NP were characterized by absorption and fluorescence spectroscopy. The cytotoxicity was investigated in Caco -2 cells by determining the cell viability and proliferation. The EC50 values for the Si NP ranged from 20 μg/l for the amine-terminated Si NP, via 550–850 μg/l for the azide-terminated Si NP to non-toxic (no measureable EC50) for the carboxylic acid - terminated Si NP. These results indicated a trend in cytotoxicity, depending on surface charge, i.e. that positively charged Si NP we re more cytotoxic than negatively charged Si NP. Interestingly, it appeared that the cytotoxicity of the amine terminated Si NP (Si NP-NH2) depended strongly on the presence of fetal calf serum (FCS) in the medium.  ",
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author = "S. Bhattacharjee",
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language = "English",
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Bhattacharjee, S 2012, 'Development and hazard assessment of nanoparticles', Doctor of Philosophy, Wageningen University, S.l..

Development and hazard assessment of nanoparticles. / Bhattacharjee, S.

S.l. : s.n., 2012. 240 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Development and hazard assessment of nanoparticles

AU - Bhattacharjee, S.

N1 - WU thesis 5286

PY - 2012

Y1 - 2012

N2 - A series of highly monodisperse silicon nanoparticles (Si NP) with either positively (amine), neutral (azide) or negatively (carboxylic acid) charged covalently attached organic monolayers were synthesized and investigated for their cytotoxicity. Infrared data confirmed the presence of these covalently attached surface groups. The Si NP were characterized by absorption and fluorescence spectroscopy. The cytotoxicity was investigated in Caco -2 cells by determining the cell viability and proliferation. The EC50 values for the Si NP ranged from 20 μg/l for the amine-terminated Si NP, via 550–850 μg/l for the azide-terminated Si NP to non-toxic (no measureable EC50) for the carboxylic acid - terminated Si NP. These results indicated a trend in cytotoxicity, depending on surface charge, i.e. that positively charged Si NP we re more cytotoxic than negatively charged Si NP. Interestingly, it appeared that the cytotoxicity of the amine terminated Si NP (Si NP-NH2) depended strongly on the presence of fetal calf serum (FCS) in the medium.  

AB - A series of highly monodisperse silicon nanoparticles (Si NP) with either positively (amine), neutral (azide) or negatively (carboxylic acid) charged covalently attached organic monolayers were synthesized and investigated for their cytotoxicity. Infrared data confirmed the presence of these covalently attached surface groups. The Si NP were characterized by absorption and fluorescence spectroscopy. The cytotoxicity was investigated in Caco -2 cells by determining the cell viability and proliferation. The EC50 values for the Si NP ranged from 20 μg/l for the amine-terminated Si NP, via 550–850 μg/l for the azide-terminated Si NP to non-toxic (no measureable EC50) for the carboxylic acid - terminated Si NP. These results indicated a trend in cytotoxicity, depending on surface charge, i.e. that positively charged Si NP we re more cytotoxic than negatively charged Si NP. Interestingly, it appeared that the cytotoxicity of the amine terminated Si NP (Si NP-NH2) depended strongly on the presence of fetal calf serum (FCS) in the medium.  

KW - nanotechnologie

KW - deeltjes

KW - gezondheidsgevaren

KW - risicoschatting

KW - cytotoxiciteit

KW - ratten

KW - biologische beschikbaarheid

KW - werkwijze

KW - nanotechnology

KW - particles

KW - health hazards

KW - risk assessment

KW - cytotoxicity

KW - rats

KW - bioavailability

KW - mode of action

M3 - internal PhD, WU

SN - 9789461733481

PB - s.n.

CY - S.l.

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Bhattacharjee S. Development and hazard assessment of nanoparticles. S.l.: s.n., 2012. 240 p.