Development and evaluation of two TaqMan assays for generic detection of Dickeya species

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Abstract

Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a background of potato DNA. The assays were evaluated with DNA extracted from potato tuber extracts, in which different Dickeya species can be present. Results of both assays were highly similar indicating that they can be used reliably in potato seed testing programs.

Original languageEnglish
Pages (from-to)311-316
Number of pages6
JournalEuropean Journal of Plant Pathology
Volume156
Early online date22 Nov 2019
DOIs
Publication statusPublished - Jan 2020

Keywords

  • Accuracy
  • Blackleg
  • Diagnostics
  • Refused sample material
  • Seed potato
  • Sensitivity
  • Specificity

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