Development and evaluation of two TaqMan assays for generic detection of Dickeya species

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a background of potato DNA. The assays were evaluated with DNA extracted from potato tuber extracts, in which different Dickeya species can be present. Results of both assays were highly similar indicating that they can be used reliably in potato seed testing programs.

Original languageEnglish
Pages (from-to)311-316
Number of pages6
JournalEuropean Journal of Plant Pathology
Volume156
Early online date22 Nov 2019
DOIs
Publication statusPublished - Jan 2020

Fingerprint

Dickeya
assays
DNA
Erwinia chrysanthemi
potatoes
seed tubers
tubers
extracts

Keywords

  • Accuracy
  • Blackleg
  • Diagnostics
  • Refused sample material
  • Seed potato
  • Sensitivity
  • Specificity

Cite this

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title = "Development and evaluation of two TaqMan assays for generic detection of Dickeya species",
abstract = "Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a background of potato DNA. The assays were evaluated with DNA extracted from potato tuber extracts, in which different Dickeya species can be present. Results of both assays were highly similar indicating that they can be used reliably in potato seed testing programs.",
keywords = "Accuracy, Blackleg, Diagnostics, Refused sample material, Seed potato, Sensitivity, Specificity",
author = "Carolien Zijlstra and {De Haas}, {Lia Groenenboom} and Marjon Krijger and Els Verstappen and Sven Warris and {de Haan}, Eisse and {van der Wolf}, Jan",
year = "2020",
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AU - Zijlstra, Carolien

AU - De Haas, Lia Groenenboom

AU - Krijger, Marjon

AU - Verstappen, Els

AU - Warris, Sven

AU - de Haan, Eisse

AU - van der Wolf, Jan

PY - 2020/1

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N2 - Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a background of potato DNA. The assays were evaluated with DNA extracted from potato tuber extracts, in which different Dickeya species can be present. Results of both assays were highly similar indicating that they can be used reliably in potato seed testing programs.

AB - Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a background of potato DNA. The assays were evaluated with DNA extracted from potato tuber extracts, in which different Dickeya species can be present. Results of both assays were highly similar indicating that they can be used reliably in potato seed testing programs.

KW - Accuracy

KW - Blackleg

KW - Diagnostics

KW - Refused sample material

KW - Seed potato

KW - Sensitivity

KW - Specificity

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VL - 156

SP - 311

EP - 316

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

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