Development and characterisation of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume

M. Zavodna; P. Arens; B. Vosman; P.J. van Dijk; J.M.M. van Damme

doi:10.1046/j.1471-8286.2002.00319.x

Development and characterisation of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume

M. Zavodna, P. Arens, B. Vosman, P.J. van Dijk, J.M.M. van Damme

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

Microsatellite markers for the pollinator fig wasp Liporrhopalum tentacularis were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. A subset of 31 positive clones was sequenced and primers were designed. Eleven primer pairs produced polymorphic amplification products in L. tentacularis. Eight markers gave unambiguously scorable patterns and were further characterized on 29 individuals collected from different fruits of the dioecious host fig Ficus montana in Indonesia. Three to 19 alleles per locus were detected in this set of samples. The observed heterozygosity ranged between 0.10 and 0.55

Original language	English
Pages (from-to)	569-571
Journal	Molecular Ecology Notes
Volume	2
DOIs	https://doi.org/10.1046/j.1471-8286.2002.00319.x
Publication status	Published - 2002

Keywords

Agaonidae
Breeding system
Ficus
Habitat fragmentation
Krakatau islands
Simple sequence repeat (SSR)

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10.1046/j.1471-8286.2002.00319.x

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title = "Development and characterisation of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume",

abstract = "Microsatellite markers for the pollinator fig wasp Liporrhopalum tentacularis were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. A subset of 31 positive clones was sequenced and primers were designed. Eleven primer pairs produced polymorphic amplification products in L. tentacularis. Eight markers gave unambiguously scorable patterns and were further characterized on 29 individuals collected from different fruits of the dioecious host fig Ficus montana in Indonesia. Three to 19 alleles per locus were detected in this set of samples. The observed heterozygosity ranged between 0.10 and 0.55",

keywords = "Agaonidae, Breeding system, Ficus, Habitat fragmentation, Krakatau islands, Simple sequence repeat (SSR)",

author = "M. Zavodna and P. Arens and B. Vosman and {van Dijk}, P.J. and {van Damme}, J.M.M.",

year = "2002",

doi = "10.1046/j.1471-8286.2002.00319.x",

language = "English",

volume = "2",

pages = "569--571",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Blackwell",

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TY - JOUR

T1 - Development and characterisation of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume

AU - Zavodna, M.

AU - Arens, P.

AU - Vosman, B.

AU - van Dijk, P.J.

AU - van Damme, J.M.M.

PY - 2002

Y1 - 2002

N2 - Microsatellite markers for the pollinator fig wasp Liporrhopalum tentacularis were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. A subset of 31 positive clones was sequenced and primers were designed. Eleven primer pairs produced polymorphic amplification products in L. tentacularis. Eight markers gave unambiguously scorable patterns and were further characterized on 29 individuals collected from different fruits of the dioecious host fig Ficus montana in Indonesia. Three to 19 alleles per locus were detected in this set of samples. The observed heterozygosity ranged between 0.10 and 0.55

AB - Microsatellite markers for the pollinator fig wasp Liporrhopalum tentacularis were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. A subset of 31 positive clones was sequenced and primers were designed. Eleven primer pairs produced polymorphic amplification products in L. tentacularis. Eight markers gave unambiguously scorable patterns and were further characterized on 29 individuals collected from different fruits of the dioecious host fig Ficus montana in Indonesia. Three to 19 alleles per locus were detected in this set of samples. The observed heterozygosity ranged between 0.10 and 0.55

KW - Agaonidae

KW - Breeding system

KW - Ficus

KW - Habitat fragmentation

KW - Krakatau islands

KW - Simple sequence repeat (SSR)

U2 - 10.1046/j.1471-8286.2002.00319.x

DO - 10.1046/j.1471-8286.2002.00319.x

M3 - Article

SN - 1471-8278

VL - 2

SP - 569

EP - 571

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

ER -

Development and characterisation of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume

Abstract

Keywords

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