Development and characterisation of 140 new microsatellites in apple (Malus x domestica Borkh.)

R. Liebhard, L. Gianfranceschi, B. Koller, C.D. Ryder, R. Tarchini, W.E. van de Weg, C. Gessler

    Research output: Contribution to journalArticleAcademicpeer-review

    437 Citations (Scopus)

    Abstract

    The availability of suitable genetic markers is essential to efficiently select and breed apple varieties of high quality and with multiple disease resistances. Microsatellites (simple sequence repeats, SSR) are very useful in this respect since they are codominant, highly polymorphic, abundant and reliably reproducible. Over 140 new SSR markers have been developed in apple and tested on a panel of 7 cultivars and 1 breeding selection. Their high level of polymorphism is expressed with an average of 6.1 alleles per locus and an average heterozygosity (H) of 0.74. Of all SSR markers, 115 have been positioned on a genetic linkage map of the cross Fiesta × Discovery. As a result, all 17 linkage groups, corresponding to the 17 chromosomes of apple, were identified. Each chromosome carries at least two SSR markers, allowing the alignment of any apple molecular marker map both with regard to identification as well as to orientation of the linkage groups. To test the degree of conservation of the SSR flanking regions and the transferability of the SSR markers to other Rosaceae species, 15 primer pairs were tested on a series of Maloideae and Amygdaloideae species. The usefulness of the newly developed microsatellites in genetic mapping is demonstrated by means of the genetic linkage map. The possibility of constructing a global apple linkage map and the impact of such a number of microsatellite markers on gene and QTL mapping is discussed
    Original languageEnglish
    Pages (from-to)217-241
    JournalMolecular Breeding
    Volume10
    Issue number4
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Malus
    Microsatellite Repeats
    apples
    microsatellite repeats
    chromosome mapping
    Genetic Linkage
    linkage groups
    Rosaceae
    chromosomes
    Chromosomes, Human, Pair 17
    genetic markers
    Disease Resistance
    Chromosome Mapping
    Genetic Markers
    Breeding
    disease resistance
    quantitative trait loci
    heterozygosity
    Chromosomes
    Alleles

    Keywords

    • scab resistance
    • linkage map
    • genetic diversity
    • aflp markers
    • ssr markers
    • rapd
    • vf
    • identification
    • polymorphism
    • construction

    Cite this

    Liebhard, R., Gianfranceschi, L., Koller, B., Ryder, C. D., Tarchini, R., van de Weg, W. E., & Gessler, C. (2002). Development and characterisation of 140 new microsatellites in apple (Malus x domestica Borkh.). Molecular Breeding, 10(4), 217-241. https://doi.org/10.1023/A:1020525906332
    Liebhard, R. ; Gianfranceschi, L. ; Koller, B. ; Ryder, C.D. ; Tarchini, R. ; van de Weg, W.E. ; Gessler, C. / Development and characterisation of 140 new microsatellites in apple (Malus x domestica Borkh.). In: Molecular Breeding. 2002 ; Vol. 10, No. 4. pp. 217-241.
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    abstract = "The availability of suitable genetic markers is essential to efficiently select and breed apple varieties of high quality and with multiple disease resistances. Microsatellites (simple sequence repeats, SSR) are very useful in this respect since they are codominant, highly polymorphic, abundant and reliably reproducible. Over 140 new SSR markers have been developed in apple and tested on a panel of 7 cultivars and 1 breeding selection. Their high level of polymorphism is expressed with an average of 6.1 alleles per locus and an average heterozygosity (H) of 0.74. Of all SSR markers, 115 have been positioned on a genetic linkage map of the cross Fiesta × Discovery. As a result, all 17 linkage groups, corresponding to the 17 chromosomes of apple, were identified. Each chromosome carries at least two SSR markers, allowing the alignment of any apple molecular marker map both with regard to identification as well as to orientation of the linkage groups. To test the degree of conservation of the SSR flanking regions and the transferability of the SSR markers to other Rosaceae species, 15 primer pairs were tested on a series of Maloideae and Amygdaloideae species. The usefulness of the newly developed microsatellites in genetic mapping is demonstrated by means of the genetic linkage map. The possibility of constructing a global apple linkage map and the impact of such a number of microsatellite markers on gene and QTL mapping is discussed",
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    author = "R. Liebhard and L. Gianfranceschi and B. Koller and C.D. Ryder and R. Tarchini and {van de Weg}, W.E. and C. Gessler",
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    Liebhard, R, Gianfranceschi, L, Koller, B, Ryder, CD, Tarchini, R, van de Weg, WE & Gessler, C 2002, 'Development and characterisation of 140 new microsatellites in apple (Malus x domestica Borkh.)', Molecular Breeding, vol. 10, no. 4, pp. 217-241. https://doi.org/10.1023/A:1020525906332

    Development and characterisation of 140 new microsatellites in apple (Malus x domestica Borkh.). / Liebhard, R.; Gianfranceschi, L.; Koller, B.; Ryder, C.D.; Tarchini, R.; van de Weg, W.E.; Gessler, C.

    In: Molecular Breeding, Vol. 10, No. 4, 2002, p. 217-241.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Development and characterisation of 140 new microsatellites in apple (Malus x domestica Borkh.)

    AU - Liebhard, R.

    AU - Gianfranceschi, L.

    AU - Koller, B.

    AU - Ryder, C.D.

    AU - Tarchini, R.

    AU - van de Weg, W.E.

    AU - Gessler, C.

    PY - 2002

    Y1 - 2002

    N2 - The availability of suitable genetic markers is essential to efficiently select and breed apple varieties of high quality and with multiple disease resistances. Microsatellites (simple sequence repeats, SSR) are very useful in this respect since they are codominant, highly polymorphic, abundant and reliably reproducible. Over 140 new SSR markers have been developed in apple and tested on a panel of 7 cultivars and 1 breeding selection. Their high level of polymorphism is expressed with an average of 6.1 alleles per locus and an average heterozygosity (H) of 0.74. Of all SSR markers, 115 have been positioned on a genetic linkage map of the cross Fiesta × Discovery. As a result, all 17 linkage groups, corresponding to the 17 chromosomes of apple, were identified. Each chromosome carries at least two SSR markers, allowing the alignment of any apple molecular marker map both with regard to identification as well as to orientation of the linkage groups. To test the degree of conservation of the SSR flanking regions and the transferability of the SSR markers to other Rosaceae species, 15 primer pairs were tested on a series of Maloideae and Amygdaloideae species. The usefulness of the newly developed microsatellites in genetic mapping is demonstrated by means of the genetic linkage map. The possibility of constructing a global apple linkage map and the impact of such a number of microsatellite markers on gene and QTL mapping is discussed

    AB - The availability of suitable genetic markers is essential to efficiently select and breed apple varieties of high quality and with multiple disease resistances. Microsatellites (simple sequence repeats, SSR) are very useful in this respect since they are codominant, highly polymorphic, abundant and reliably reproducible. Over 140 new SSR markers have been developed in apple and tested on a panel of 7 cultivars and 1 breeding selection. Their high level of polymorphism is expressed with an average of 6.1 alleles per locus and an average heterozygosity (H) of 0.74. Of all SSR markers, 115 have been positioned on a genetic linkage map of the cross Fiesta × Discovery. As a result, all 17 linkage groups, corresponding to the 17 chromosomes of apple, were identified. Each chromosome carries at least two SSR markers, allowing the alignment of any apple molecular marker map both with regard to identification as well as to orientation of the linkage groups. To test the degree of conservation of the SSR flanking regions and the transferability of the SSR markers to other Rosaceae species, 15 primer pairs were tested on a series of Maloideae and Amygdaloideae species. The usefulness of the newly developed microsatellites in genetic mapping is demonstrated by means of the genetic linkage map. The possibility of constructing a global apple linkage map and the impact of such a number of microsatellite markers on gene and QTL mapping is discussed

    KW - scab resistance

    KW - linkage map

    KW - genetic diversity

    KW - aflp markers

    KW - ssr markers

    KW - rapd

    KW - vf

    KW - identification

    KW - polymorphism

    KW - construction

    U2 - 10.1023/A:1020525906332

    DO - 10.1023/A:1020525906332

    M3 - Article

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    SP - 217

    EP - 241

    JO - Molecular Breeding

    JF - Molecular Breeding

    SN - 1380-3743

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    ER -