Development and application of genetically uniform strains of common carp (Cyprinus carpio L.)

A.B.J. Bongers

Research output: Thesisinternal PhD, WU

Abstract

<p>In this thesis, the development of genetically uniform strains of common carp, <em>Cyprinus carpio</em> L. is described. As in research on mammals, the use of genetically uniform fish could increase the quality (replicability, reproducability and repeatability) of experiments. Inbreeding was done by gynogenetic and androgenetic reproduction. With these methods, fully homozygous. offspring are produced in only one generation. The principle of these reproduction techniques is to eliminate the genetic contribution of one of the parents by (UV) irradiating the sexual products, followed by artificially inducing diploidy by physical shocks in the developing zygotes. Inbred strains, congenic strains and F1 hybrids were produced. Congenic strains were used to study sex determination in carp. Inbred strains and F1 hybrids were used to study the genetic control of gonad development after a quantitative genetic model had been developed to facilitate estimation of genetic variance. We show that genetically uniform carp strains are suitable as experimental animal model in genetic research and in bioassays (toxicological research). It is obvious that other research areas could also profit from genetically uniform carp strains.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Richter, C.J.J., Promotor, External person
  • van Muiswinkel, W.B., Promotor, External person
  • Komen, Hans, Promotor
Award date14 Jan 1997
Place of PublicationS.l.
Publisher
Print ISBNs9789054856252
Publication statusPublished - 1997

Fingerprint

Cyprinus carpio
carp
quantitative genetics
zygote
genetic variance
repeatability
inbreeding
profits and margins
laboratory animals
gonads
diploidy
bioassays
animal models
mammals
gender
methodology
fish

Keywords

  • cyprinidae
  • carp
  • inbreeding
  • inbred lines
  • homozygotes
  • homozygosity
  • laboratory animals
  • laboratory methods
  • techniques
  • genetic engineering
  • recombinant dna
  • genetic variation
  • inheritance
  • breeds
  • races
  • experiments
  • embryology
  • parthenogenesis
  • animal cloning

Cite this

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title = "Development and application of genetically uniform strains of common carp (Cyprinus carpio L.)",
abstract = "In this thesis, the development of genetically uniform strains of common carp, Cyprinus carpio L. is described. As in research on mammals, the use of genetically uniform fish could increase the quality (replicability, reproducability and repeatability) of experiments. Inbreeding was done by gynogenetic and androgenetic reproduction. With these methods, fully homozygous. offspring are produced in only one generation. The principle of these reproduction techniques is to eliminate the genetic contribution of one of the parents by (UV) irradiating the sexual products, followed by artificially inducing diploidy by physical shocks in the developing zygotes. Inbred strains, congenic strains and F1 hybrids were produced. Congenic strains were used to study sex determination in carp. Inbred strains and F1 hybrids were used to study the genetic control of gonad development after a quantitative genetic model had been developed to facilitate estimation of genetic variance. We show that genetically uniform carp strains are suitable as experimental animal model in genetic research and in bioassays (toxicological research). It is obvious that other research areas could also profit from genetically uniform carp strains.",
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author = "A.B.J. Bongers",
note = "WU thesis 2203 Proefschrift Wageningen",
year = "1997",
language = "English",
isbn = "9789054856252",
publisher = "Bongers",

}

Development and application of genetically uniform strains of common carp (Cyprinus carpio L.). / Bongers, A.B.J.

S.l. : Bongers, 1997. 159 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Development and application of genetically uniform strains of common carp (Cyprinus carpio L.)

AU - Bongers, A.B.J.

N1 - WU thesis 2203 Proefschrift Wageningen

PY - 1997

Y1 - 1997

N2 - In this thesis, the development of genetically uniform strains of common carp, Cyprinus carpio L. is described. As in research on mammals, the use of genetically uniform fish could increase the quality (replicability, reproducability and repeatability) of experiments. Inbreeding was done by gynogenetic and androgenetic reproduction. With these methods, fully homozygous. offspring are produced in only one generation. The principle of these reproduction techniques is to eliminate the genetic contribution of one of the parents by (UV) irradiating the sexual products, followed by artificially inducing diploidy by physical shocks in the developing zygotes. Inbred strains, congenic strains and F1 hybrids were produced. Congenic strains were used to study sex determination in carp. Inbred strains and F1 hybrids were used to study the genetic control of gonad development after a quantitative genetic model had been developed to facilitate estimation of genetic variance. We show that genetically uniform carp strains are suitable as experimental animal model in genetic research and in bioassays (toxicological research). It is obvious that other research areas could also profit from genetically uniform carp strains.

AB - In this thesis, the development of genetically uniform strains of common carp, Cyprinus carpio L. is described. As in research on mammals, the use of genetically uniform fish could increase the quality (replicability, reproducability and repeatability) of experiments. Inbreeding was done by gynogenetic and androgenetic reproduction. With these methods, fully homozygous. offspring are produced in only one generation. The principle of these reproduction techniques is to eliminate the genetic contribution of one of the parents by (UV) irradiating the sexual products, followed by artificially inducing diploidy by physical shocks in the developing zygotes. Inbred strains, congenic strains and F1 hybrids were produced. Congenic strains were used to study sex determination in carp. Inbred strains and F1 hybrids were used to study the genetic control of gonad development after a quantitative genetic model had been developed to facilitate estimation of genetic variance. We show that genetically uniform carp strains are suitable as experimental animal model in genetic research and in bioassays (toxicological research). It is obvious that other research areas could also profit from genetically uniform carp strains.

KW - cyprinidae

KW - karper

KW - inteelt

KW - inteeltlijnen

KW - homozygoten

KW - homozygotie

KW - laboratoriumdieren

KW - laboratoriummethoden

KW - technieken

KW - genetische modificatie

KW - recombinant dna

KW - genetische variatie

KW - overerving

KW - rassen (dieren)

KW - rassen (taxonomisch)

KW - experimenten

KW - embryologie

KW - parthenogenese

KW - dierkloneren

KW - cyprinidae

KW - carp

KW - inbreeding

KW - inbred lines

KW - homozygotes

KW - homozygosity

KW - laboratory animals

KW - laboratory methods

KW - techniques

KW - genetic engineering

KW - recombinant dna

KW - genetic variation

KW - inheritance

KW - breeds

KW - races

KW - experiments

KW - embryology

KW - parthenogenesis

KW - animal cloning

M3 - internal PhD, WU

SN - 9789054856252

PB - Bongers

CY - S.l.

ER -