Developing microalgal oil production for an outdoor photobioreactor

Niels Henrik Norsker*, Maria Cuaresma, Pauliina Uronen, Maria J. Barbosa, René Wijffels

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


In this paper the preparations are described to develop a production of oil rich microalgal biomass under south European conditions. Ten microalgal species were compared in shake flasks in an incubator for potential for oil production. Potential oil production capacity was assayed as maximum total fatty acid (TFA) concentration and volumetric TFA productivity. TFA concentration ranged from 5 to 40% DW while TFA productivity rate ranged from 0 to 204 mg TFA L−1 day−1. To control the oil enrichment process in the outdoor microalgal batch culture, a quadratic equation was proposed, predicting the TFA concentration based on biomass inverse nitrogen quota. A concentrated substrate was developed to add to sea water, made from natural sea-salt and tap water.

Original languageEnglish
JournalJournal of Applied Phycology
Publication statusE-pub ahead of print - 12 Mar 2021


  • Algal production
  • Fatty acids
  • Oil
  • Oil enhancement
  • Strains

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