Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi

M.G. Teklu, T.H. Been, C.H. Schomaker, L.P.G. Molendijk, T.G. van Beers, Annelies Beniers

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

In The Netherlands resistance genes against Meloidogyne chitwoodi became available as a result of the DREAM (EU QLRT-1999-1462) project. Several Dutch potato breeders have used these genes to develop potato genotypes containing a single resistance gene against M. chitwoodi. Some of these genes also provide resistance against other Meloidogyne species. Currently, a research project has been initiated by Dutch breeders to investigate the impact of these genotypes on the population dynamics, yield loss and tuber infestation with M. chitwoodi. Also, the development of a simple and cheap resistance test, as has been realised for PCN resistant potatoes, is pursued. At this moment three large experiments have been conducted to obtain the required basic information. Ten potato genotypes were screened for their resistance against M. chitwoodi. The susceptible potato cv. Désirée was used as a susceptible control. Potato genotypes were grown in 5 and 10 l pots in an artificial soil at ranges of initial nematode densities (Pi), e.g. from 0, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64 to 128 (g of dry soil)-1, to enable fitting population dynamical and yield models and estimating relative susceptibility (rs), tolerance, yield loss and tuber quality, expressed as root-knot index (RKI). The partial resistance of the genotypes with a single resistance gene was very high > 99%, except for two genotypes with non-M. chitwoodi resistance genes (<50%). Three of the resistant genotypes also showed a remarkable tolerance for high initial population densities of M. chitwoodi. A marked improvement of tuber quality was found in eight genotypes with M. chitwoodi resistance, with RKI value below 10, the minimum threshold for acceptance of ware potatoes for industrial processing. So far, on nine genotypes screened for resistance, the population dynamical models used to describe relative susceptibility, fitted well. From the comparison of the population dynamics of both the susceptible and resistant varieties it can be concluded that downscaling of the screening method is feasible.
Original languageEnglish
Title of host publicationThe 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012
Pages221-221
Publication statusPublished - 2012
EventThe 31th International Symposium of the European Society of Nematologists - Adana
Duration: 23 Sep 201227 Sep 2012

Conference

ConferenceThe 31th International Symposium of the European Society of Nematologists
CityAdana
Period23/09/1227/09/12

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Meloidogyne chitwoodi
potatoes
screening
genotype
tubers
genes
methodology
population dynamics
Meloidogyne
research projects
processing technology
soil
Netherlands
Nematoda
testing

Cite this

Teklu, M. G., Been, T. H., Schomaker, C. H., Molendijk, L. P. G., van Beers, T. G., & Beniers, A. (2012). Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi. In The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012 (pp. 221-221)
Teklu, M.G. ; Been, T.H. ; Schomaker, C.H. ; Molendijk, L.P.G. ; van Beers, T.G. ; Beniers, Annelies. / Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi. The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012. 2012. pp. 221-221
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title = "Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi",
abstract = "In The Netherlands resistance genes against Meloidogyne chitwoodi became available as a result of the DREAM (EU QLRT-1999-1462) project. Several Dutch potato breeders have used these genes to develop potato genotypes containing a single resistance gene against M. chitwoodi. Some of these genes also provide resistance against other Meloidogyne species. Currently, a research project has been initiated by Dutch breeders to investigate the impact of these genotypes on the population dynamics, yield loss and tuber infestation with M. chitwoodi. Also, the development of a simple and cheap resistance test, as has been realised for PCN resistant potatoes, is pursued. At this moment three large experiments have been conducted to obtain the required basic information. Ten potato genotypes were screened for their resistance against M. chitwoodi. The susceptible potato cv. D{\'e}sir{\'e}e was used as a susceptible control. Potato genotypes were grown in 5 and 10 l pots in an artificial soil at ranges of initial nematode densities (Pi), e.g. from 0, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64 to 128 (g of dry soil)-1, to enable fitting population dynamical and yield models and estimating relative susceptibility (rs), tolerance, yield loss and tuber quality, expressed as root-knot index (RKI). The partial resistance of the genotypes with a single resistance gene was very high > 99{\%}, except for two genotypes with non-M. chitwoodi resistance genes (<50{\%}). Three of the resistant genotypes also showed a remarkable tolerance for high initial population densities of M. chitwoodi. A marked improvement of tuber quality was found in eight genotypes with M. chitwoodi resistance, with RKI value below 10, the minimum threshold for acceptance of ware potatoes for industrial processing. So far, on nine genotypes screened for resistance, the population dynamical models used to describe relative susceptibility, fitted well. From the comparison of the population dynamics of both the susceptible and resistant varieties it can be concluded that downscaling of the screening method is feasible.",
author = "M.G. Teklu and T.H. Been and C.H. Schomaker and L.P.G. Molendijk and {van Beers}, T.G. and Annelies Beniers",
year = "2012",
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booktitle = "The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012",

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Teklu, MG, Been, TH, Schomaker, CH, Molendijk, LPG, van Beers, TG & Beniers, A 2012, Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi. in The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012. pp. 221-221, The 31th International Symposium of the European Society of Nematologists, Adana, 23/09/12.

Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi. / Teklu, M.G.; Been, T.H.; Schomaker, C.H.; Molendijk, L.P.G.; van Beers, T.G.; Beniers, Annelies.

The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012. 2012. p. 221-221.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi

AU - Teklu, M.G.

AU - Been, T.H.

AU - Schomaker, C.H.

AU - Molendijk, L.P.G.

AU - van Beers, T.G.

AU - Beniers, Annelies

PY - 2012

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N2 - In The Netherlands resistance genes against Meloidogyne chitwoodi became available as a result of the DREAM (EU QLRT-1999-1462) project. Several Dutch potato breeders have used these genes to develop potato genotypes containing a single resistance gene against M. chitwoodi. Some of these genes also provide resistance against other Meloidogyne species. Currently, a research project has been initiated by Dutch breeders to investigate the impact of these genotypes on the population dynamics, yield loss and tuber infestation with M. chitwoodi. Also, the development of a simple and cheap resistance test, as has been realised for PCN resistant potatoes, is pursued. At this moment three large experiments have been conducted to obtain the required basic information. Ten potato genotypes were screened for their resistance against M. chitwoodi. The susceptible potato cv. Désirée was used as a susceptible control. Potato genotypes were grown in 5 and 10 l pots in an artificial soil at ranges of initial nematode densities (Pi), e.g. from 0, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64 to 128 (g of dry soil)-1, to enable fitting population dynamical and yield models and estimating relative susceptibility (rs), tolerance, yield loss and tuber quality, expressed as root-knot index (RKI). The partial resistance of the genotypes with a single resistance gene was very high > 99%, except for two genotypes with non-M. chitwoodi resistance genes (<50%). Three of the resistant genotypes also showed a remarkable tolerance for high initial population densities of M. chitwoodi. A marked improvement of tuber quality was found in eight genotypes with M. chitwoodi resistance, with RKI value below 10, the minimum threshold for acceptance of ware potatoes for industrial processing. So far, on nine genotypes screened for resistance, the population dynamical models used to describe relative susceptibility, fitted well. From the comparison of the population dynamics of both the susceptible and resistant varieties it can be concluded that downscaling of the screening method is feasible.

AB - In The Netherlands resistance genes against Meloidogyne chitwoodi became available as a result of the DREAM (EU QLRT-1999-1462) project. Several Dutch potato breeders have used these genes to develop potato genotypes containing a single resistance gene against M. chitwoodi. Some of these genes also provide resistance against other Meloidogyne species. Currently, a research project has been initiated by Dutch breeders to investigate the impact of these genotypes on the population dynamics, yield loss and tuber infestation with M. chitwoodi. Also, the development of a simple and cheap resistance test, as has been realised for PCN resistant potatoes, is pursued. At this moment three large experiments have been conducted to obtain the required basic information. Ten potato genotypes were screened for their resistance against M. chitwoodi. The susceptible potato cv. Désirée was used as a susceptible control. Potato genotypes were grown in 5 and 10 l pots in an artificial soil at ranges of initial nematode densities (Pi), e.g. from 0, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64 to 128 (g of dry soil)-1, to enable fitting population dynamical and yield models and estimating relative susceptibility (rs), tolerance, yield loss and tuber quality, expressed as root-knot index (RKI). The partial resistance of the genotypes with a single resistance gene was very high > 99%, except for two genotypes with non-M. chitwoodi resistance genes (<50%). Three of the resistant genotypes also showed a remarkable tolerance for high initial population densities of M. chitwoodi. A marked improvement of tuber quality was found in eight genotypes with M. chitwoodi resistance, with RKI value below 10, the minimum threshold for acceptance of ware potatoes for industrial processing. So far, on nine genotypes screened for resistance, the population dynamical models used to describe relative susceptibility, fitted well. From the comparison of the population dynamics of both the susceptible and resistant varieties it can be concluded that downscaling of the screening method is feasible.

M3 - Abstract

SP - 221

EP - 221

BT - The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012

ER -

Teklu MG, Been TH, Schomaker CH, Molendijk LPG, van Beers TG, Beniers A. Developing methodology for screening the relative susceptibility of potatoes with resistance against M. chitwoodi. In The 31th International Symposium of the European Society of Nematologists, Adana, Turkey, 23-27 September 2012. 2012. p. 221-221