Determination of triacylglycerols in milk fat from different species using UPLC–Q-TOF–MS

Emad Karrar; Isam A. Mohamed Ahmed; Thom Huppertz; Fatih Oz; Wei Wei; Xingguo Wang

doi:10.1016/j.idairyj.2022.105405

Determination of triacylglycerols in milk fat from different species using UPLC–Q-TOF–MS

Emad Karrar, Isam A. Mohamed Ahmed, Thom Huppertz, Fatih Oz, Wei Wei, Xingguo Wang^*

^*Corresponding author for this work

Food Quality and Design

Research output: Contribution to journal › Article › Academic › peer-review

4 Citations (Scopus)

Abstract

Triacylglycerols (TAGs) are the major component of milk lipids and play important technological, nutritional and physiological roles. In this study, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF–MS) was used to characterise TAGs in cow, yak, donkey, goat, and camel milk fat. TAG separation based on partition number was achieved through an acetonitrile-isopropanol gradient elution. A total of 47, 52, 61, 75, and 87 TAGs were identified in camel, goat, donkey, cow, and yak milk fat, respectively, by UPLC–Q-TOF–MS. The most abundant TAG species in cow, yak, donkey, goat, and camel milk fat was 38:1, 40:3, 38:1, 40:1, and 52:2, respectively. TAG analysis using UPLC–Q-TOF–MS proved its potential to analyse different kinds of milk fat. This work contributes to a better understanding of the TAGs found in milk from various species.

Original language	English
Article number	105405
Journal	International Dairy Journal
Volume	133
DOIs	https://doi.org/10.1016/j.idairyj.2022.105405
Publication status	Published - Oct 2022

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@article{129f7702625942d48c6fdfdcb05eaaf3,

title = "Determination of triacylglycerols in milk fat from different species using UPLC–Q-TOF–MS",

abstract = "Triacylglycerols (TAGs) are the major component of milk lipids and play important technological, nutritional and physiological roles. In this study, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF–MS) was used to characterise TAGs in cow, yak, donkey, goat, and camel milk fat. TAG separation based on partition number was achieved through an acetonitrile-isopropanol gradient elution. A total of 47, 52, 61, 75, and 87 TAGs were identified in camel, goat, donkey, cow, and yak milk fat, respectively, by UPLC–Q-TOF–MS. The most abundant TAG species in cow, yak, donkey, goat, and camel milk fat was 38:1, 40:3, 38:1, 40:1, and 52:2, respectively. TAG analysis using UPLC–Q-TOF–MS proved its potential to analyse different kinds of milk fat. This work contributes to a better understanding of the TAGs found in milk from various species.",

author = "Emad Karrar and {Mohamed Ahmed}, {Isam A.} and Thom Huppertz and Fatih Oz and Wei Wei and Xingguo Wang",

year = "2022",

month = oct,

doi = "10.1016/j.idairyj.2022.105405",

language = "English",

volume = "133",

journal = "International Dairy Journal",

issn = "0958-6946",

publisher = "Elsevier",

}

TY - JOUR

T1 - Determination of triacylglycerols in milk fat from different species using UPLC–Q-TOF–MS

AU - Karrar, Emad

AU - Mohamed Ahmed, Isam A.

AU - Huppertz, Thom

AU - Oz, Fatih

AU - Wei, Wei

AU - Wang, Xingguo

PY - 2022/10

Y1 - 2022/10

N2 - Triacylglycerols (TAGs) are the major component of milk lipids and play important technological, nutritional and physiological roles. In this study, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF–MS) was used to characterise TAGs in cow, yak, donkey, goat, and camel milk fat. TAG separation based on partition number was achieved through an acetonitrile-isopropanol gradient elution. A total of 47, 52, 61, 75, and 87 TAGs were identified in camel, goat, donkey, cow, and yak milk fat, respectively, by UPLC–Q-TOF–MS. The most abundant TAG species in cow, yak, donkey, goat, and camel milk fat was 38:1, 40:3, 38:1, 40:1, and 52:2, respectively. TAG analysis using UPLC–Q-TOF–MS proved its potential to analyse different kinds of milk fat. This work contributes to a better understanding of the TAGs found in milk from various species.

AB - Triacylglycerols (TAGs) are the major component of milk lipids and play important technological, nutritional and physiological roles. In this study, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF–MS) was used to characterise TAGs in cow, yak, donkey, goat, and camel milk fat. TAG separation based on partition number was achieved through an acetonitrile-isopropanol gradient elution. A total of 47, 52, 61, 75, and 87 TAGs were identified in camel, goat, donkey, cow, and yak milk fat, respectively, by UPLC–Q-TOF–MS. The most abundant TAG species in cow, yak, donkey, goat, and camel milk fat was 38:1, 40:3, 38:1, 40:1, and 52:2, respectively. TAG analysis using UPLC–Q-TOF–MS proved its potential to analyse different kinds of milk fat. This work contributes to a better understanding of the TAGs found in milk from various species.

U2 - 10.1016/j.idairyj.2022.105405

DO - 10.1016/j.idairyj.2022.105405

M3 - Article

AN - SCOPUS:85131366983

SN - 0958-6946

VL - 133

JO - International Dairy Journal

JF - International Dairy Journal

M1 - 105405

ER -

Determination of triacylglycerols in milk fat from different species using UPLC–Q-TOF–MS

Abstract

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