Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS): First Action 2015.02

George Joseph; Darryl Sullivan; Martin Alewijn; John Austad; Joe Boison; Scott Christiansen; Jo Marie Cook; Jon De Vries; Harvey Indyk; Erik Konings; Alex Krynitsky; Tom Phillips; Bert Popping; Murali Reddy; John Wong

doi:10.5740/jaoac.int.2015.02

Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS): First Action 2015.02

George Joseph^*, Darryl Sullivan, Martin Alewijn, John Austad, Joe Boison, Scott Christiansen, Jo Marie Cook, Jon De Vries, Harvey Indyk, Erik Konings, Alex Krynitsky, Tom Phillips, Bert Popping, Murali Reddy, John Wong

^*Corresponding author for this work

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Abstract

Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS) is discussed. Samples are dissolved in water and extracted into acetone to allow precipitation of proteins. After centrifugation, the solutions are passed through an anion exchange column and eluted with acid to give free fluoroacetic acid. This acid is converted to 2-fluoro-3?-nitroacetanilide via a carbodiimide-mediated amide coupling reaction. The derivative is then subjected to SPE cleanup, eluting with t-butyl methyl ether (TBME)?n-hexane, concentrated and quantified by LC-MS/MS using derivatized isotopically substituted sodium fluoroacetate as an internal standard.

Original language	English
Pages (from-to)	1121-1126
Number of pages	6
Journal	Journal of AOAC International
Volume	98
Issue number	4
DOIs	https://doi.org/10.5740/jaoac.int.2015.02
Publication status	Published - 1 Jul 2015

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Joseph, G., Sullivan, D., Alewijn, M., Austad, J., Boison, J., Christiansen, S., Cook, J. M., De Vries, J., Indyk, H., Konings, E., Krynitsky, A., Phillips, T., Popping, B., Reddy, M., & Wong, J. (2015). Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS): First Action 2015.02. Journal of AOAC International, 98(4), 1121-1126. https://doi.org/10.5740/jaoac.int.2015.02

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title = "Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS): First Action 2015.02",

abstract = "Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS) is discussed. Samples are dissolved in water and extracted into acetone to allow precipitation of proteins. After centrifugation, the solutions are passed through an anion exchange column and eluted with acid to give free fluoroacetic acid. This acid is converted to 2-fluoro-3?-nitroacetanilide via a carbodiimide-mediated amide coupling reaction. The derivative is then subjected to SPE cleanup, eluting with t-butyl methyl ether (TBME)?n-hexane, concentrated and quantified by LC-MS/MS using derivatized isotopically substituted sodium fluoroacetate as an internal standard.",

author = "George Joseph and Darryl Sullivan and Martin Alewijn and John Austad and Joe Boison and Scott Christiansen and Cook, {Jo Marie} and {De Vries}, Jon and Harvey Indyk and Erik Konings and Alex Krynitsky and Tom Phillips and Bert Popping and Murali Reddy and John Wong",

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Joseph, G, Sullivan, D, Alewijn, M, Austad, J, Boison, J, Christiansen, S, Cook, JM, De Vries, J, Indyk, H, Konings, E, Krynitsky, A, Phillips, T, Popping, B, Reddy, M & Wong, J 2015, 'Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS): First Action 2015.02', Journal of AOAC International, vol. 98, no. 4, pp. 1121-1126. https://doi.org/10.5740/jaoac.int.2015.02

TY - JOUR

T1 - Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS): First Action 2015.02

AU - Joseph, George

AU - Sullivan, Darryl

AU - Alewijn, Martin

AU - Austad, John

AU - Boison, Joe

AU - Christiansen, Scott

AU - Cook, Jo Marie

AU - De Vries, Jon

AU - Indyk, Harvey

AU - Konings, Erik

AU - Krynitsky, Alex

AU - Phillips, Tom

AU - Popping, Bert

AU - Reddy, Murali

AU - Wong, John

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS) is discussed. Samples are dissolved in water and extracted into acetone to allow precipitation of proteins. After centrifugation, the solutions are passed through an anion exchange column and eluted with acid to give free fluoroacetic acid. This acid is converted to 2-fluoro-3?-nitroacetanilide via a carbodiimide-mediated amide coupling reaction. The derivative is then subjected to SPE cleanup, eluting with t-butyl methyl ether (TBME)?n-hexane, concentrated and quantified by LC-MS/MS using derivatized isotopically substituted sodium fluoroacetate as an internal standard.

AB - Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS) is discussed. Samples are dissolved in water and extracted into acetone to allow precipitation of proteins. After centrifugation, the solutions are passed through an anion exchange column and eluted with acid to give free fluoroacetic acid. This acid is converted to 2-fluoro-3?-nitroacetanilide via a carbodiimide-mediated amide coupling reaction. The derivative is then subjected to SPE cleanup, eluting with t-butyl methyl ether (TBME)?n-hexane, concentrated and quantified by LC-MS/MS using derivatized isotopically substituted sodium fluoroacetate as an internal standard.

U2 - 10.5740/jaoac.int.2015.02

DO - 10.5740/jaoac.int.2015.02

M3 - Article

AN - SCOPUS:84982084003

SN - 1060-3271

VL - 98

SP - 1121

EP - 1126

JO - Journal of AOAC International

JF - Journal of AOAC International

IS - 4

ER -

Determination of sodium monofluoroacetate in dairy powders by liquid chromatography tandem mass spectrometry (LC-MS/MS): First Action 2015.02

Abstract

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