Determination of sodium fluoroacetate in infant formula by liquid chromatography-tandem mass spectrometry (LC-MS/MS): First Action 2015.03

Pascal Mottier, Darryl Sullivan, Martin Alewijn, John Austad, Joe Boison, Scott Christiansen, Jo Marie Cook, Jon De Vries, Harvey Indyk, George Joseph, Erik Konings, Alex Krynitsky, Tom Phillips, Bert Popping, Murali Reddy, John Wong

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Determination of sodium fluoroacetate in infant formula by liquid chromatography-tandem mass spectrometry (LC-MS/MS)is discussed. Sodium fluoroacetate is also a naturally occurring poison found in at least 40 plants native in Australia, South and West Africa, and Brazil. Milk powder is first reconstituted in water. Liquid sample is used as such. Acetonitrile is added to precipitate proteins. After centrifugation, the supernatant is washed with hexane and then acidified with concentrated sulfuric acid. QuEChERS salts are added for phase separation and the mixture is centrifuged. The resulting supernatant is evaporated to 0.5 mL remaining volume and centrifuged before LC-MS/MS analysis in selected reaction monitoring (SRM) by electrospray ionization (ESI) in negative mode.

Original languageEnglish
Pages (from-to)1127-1134
Number of pages8
JournalJournal of AOAC International
Volume98
Issue number4
DOIs
Publication statusPublished - 1 Jul 2015

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