Determination of pectin content of eucalyptus wood

B. Coetzee, H.A. Schols, F. Wolfaardt

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Very little is known about the occurrence of pectin in wood and it is speculated that between 10 mg g-1 and 40 mg g-1 of wood consists of pectin. The present study aimed to quantify pectin in eucalyptus wood and to determine the influence of tree species, yield potential of the site, tree age class and wood tissue type on its occurrence. Wood was hydrolysed using the Saeman procedure and the neutral and acidic monosaccharides quantified with high-performance liquid chromatography (HPLC). The d-galacturonic acid was the predominant pectic monosaccharide followed by d-galactose, l-arabinose and l-rhamnose. Through the addition of all pectic monosaccharides, it was determined that eucalyptus wood contained between 15.2 mg g-1 and 25.8 mg g-1 pectin. Wood tissue type had a major influence on the total pectin content of the samples. The cambium contained the highest concentration of pectin, reflecting more active growth. These results contribute to the understanding of wood biochemistry and can be useful in the industries producing biofuels or paper.
Original languageEnglish
Pages (from-to)327-331
JournalHolzforschung
Volume65
Issue number3
DOIs
Publication statusPublished - 2011

Fingerprint

Wood
Monosaccharides
Tissue
Rhamnose
Biochemistry
Arabinose
Biofuels
pectin
High performance liquid chromatography
Galactose
Acids
Industry

Keywords

  • quince chaenomeles-japonica
  • cell
  • polysaccharides
  • degradation
  • substances
  • responses
  • fruits
  • plants
  • hplc
  • wall

Cite this

Coetzee, B. ; Schols, H.A. ; Wolfaardt, F. / Determination of pectin content of eucalyptus wood. In: Holzforschung. 2011 ; Vol. 65, No. 3. pp. 327-331.
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Determination of pectin content of eucalyptus wood. / Coetzee, B.; Schols, H.A.; Wolfaardt, F.

In: Holzforschung, Vol. 65, No. 3, 2011, p. 327-331.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Determination of pectin content of eucalyptus wood

AU - Coetzee, B.

AU - Schols, H.A.

AU - Wolfaardt, F.

PY - 2011

Y1 - 2011

N2 - Very little is known about the occurrence of pectin in wood and it is speculated that between 10 mg g-1 and 40 mg g-1 of wood consists of pectin. The present study aimed to quantify pectin in eucalyptus wood and to determine the influence of tree species, yield potential of the site, tree age class and wood tissue type on its occurrence. Wood was hydrolysed using the Saeman procedure and the neutral and acidic monosaccharides quantified with high-performance liquid chromatography (HPLC). The d-galacturonic acid was the predominant pectic monosaccharide followed by d-galactose, l-arabinose and l-rhamnose. Through the addition of all pectic monosaccharides, it was determined that eucalyptus wood contained between 15.2 mg g-1 and 25.8 mg g-1 pectin. Wood tissue type had a major influence on the total pectin content of the samples. The cambium contained the highest concentration of pectin, reflecting more active growth. These results contribute to the understanding of wood biochemistry and can be useful in the industries producing biofuels or paper.

AB - Very little is known about the occurrence of pectin in wood and it is speculated that between 10 mg g-1 and 40 mg g-1 of wood consists of pectin. The present study aimed to quantify pectin in eucalyptus wood and to determine the influence of tree species, yield potential of the site, tree age class and wood tissue type on its occurrence. Wood was hydrolysed using the Saeman procedure and the neutral and acidic monosaccharides quantified with high-performance liquid chromatography (HPLC). The d-galacturonic acid was the predominant pectic monosaccharide followed by d-galactose, l-arabinose and l-rhamnose. Through the addition of all pectic monosaccharides, it was determined that eucalyptus wood contained between 15.2 mg g-1 and 25.8 mg g-1 pectin. Wood tissue type had a major influence on the total pectin content of the samples. The cambium contained the highest concentration of pectin, reflecting more active growth. These results contribute to the understanding of wood biochemistry and can be useful in the industries producing biofuels or paper.

KW - quince chaenomeles-japonica

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KW - polysaccharides

KW - degradation

KW - substances

KW - responses

KW - fruits

KW - plants

KW - hplc

KW - wall

U2 - 10.1515/HF.2011.054

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