Determination of nivalenol in food and feed: an update

A. Malachova, H.P. van Egmond, F. Berthiller, R. Krska

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)


Based on the recent scientific opinion published by the EFSA CONTAM panel on the risks to human and animal health related to the presence of nivalenol in food and feed, this article provides an update on the determination of this Fusarium mycotoxin. After a brief introduction into the chemistry of nivalenol, chromatographic methods as well as other approaches are being discussed. Methods for the determination of nivalenol are well established and can be applied for the analysis of cereals, food, feed and biological samples. Accurate quantification of nivalenol is mostly carried out by liquid chromatography coupled with (multi-stage) mass spectrometry (MS) often within a multi-analyte approach. Some novel techniques, such as direct analysis in real time (DART) MS and electrochemical methods, have shown potential to determine nivalenol, but applications for routine measurements are not yet available. None of the currently available analytical methods has been formally validated in interlaboratory validation studies. While a certified calibrant for nivalenol is available, no matrix reference materials have been developed. Due to the scarcity of appropriate antibodies also no rapid immunochemical methods specific for nivalenol have become available
Original languageEnglish
Pages (from-to)247-255
JournalWorld Mycotoxin Journal
Issue number3
Publication statusPublished - 2014


  • tandem mass-spectrometry
  • b trichothecene mycotoxins
  • lc-ms/ms method
  • electrochemical detection
  • fusarium mycotoxins
  • multiple mycotoxins
  • quechers procedure
  • masked mycotoxins
  • cereal products
  • maize silage

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