Determination of monofluoroacetate in powdered nutritional products by derivatization with 2-nitrophenylhydrazine and LC-MS/MS: First action 2015.04

Murali Reddy, Darryl Sullivan, Martin Alewijn, John Austad, Joe Boison, Scott Christiansen, Jo Marie Cook, Jon De Vries, Harvey Indyk, George Joseph, Erik Konings, Alex Krynitsky, Tom Phillips, Bert Popping, John Wong

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Determination of monofluoroacetate in powdered nutritional products by derivatization with 2-nitrophenylhydrazine and LC-MS/MS IS discussed. Samples are prepared by dilution in water followed by protein precipitation with acetonitrile. An aliquot of the sample extract is derivatized with 2-nitrophenylhydrazine (2-NPH) in the presence of 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). This reaction achieves the coupling of the carboxyl group of monofluoroacetate to the amino group of 2-NPH with the formation of an amide bond. Samples are processed through a solid-phase extraction (SPE) step in order to exchange.

Original languageEnglish
Pages (from-to)1135-1139
Number of pages5
JournalJournal of AOAC International
Volume98
Issue number4
DOIs
Publication statusPublished - 1 Jul 2015

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