Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y

M. Verbeek; P.G.M. Piron; A.M. Dullemans; C. Cuperus; R.A.A. van der Vlugt

Abstract

Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species, which express the transmission efficiency of aphid species in relation to the efficiency of Myzus persicae, the most efficient vector of PVY.In the Netherlands, in the 1980s, aphid’s REFs were determined using aphids caught alive in the field. Thus, experiments were conducted using limited numbers of aphids and only during the potato growing season. We have now developed a system which allows us to test virus transmission whole year round, using aphid clones reared in insect chambers. Using the new system, we determined the aphids’ relative transmission efficiency factors (REFs) for six isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae showed comparable average transmission efficiencies for all isolates, and was used as an internal control to determine the REFs of 18 other aphid species. The newly determined REFs for PVYN were comparable to previously reported values. New REFs for the PVYNTN strains were overall comparable to the REFs for PVYN, except for Aphis frangulae and Schizaphis graminum. For PVYN-Wi six aphid species showed higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only A. frangulae shows a lower REF for PVYN-Wi. In addition three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determined.

Original language	English
Title of host publication	Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010
Publisher	The International Society for Plant Pathology (ISPP)
Pages	39-39
Publication status	Published - 2010
Event	Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Ithaca, USA - Duration: 20 Jun 2010 → 24 Jun 2010

Workshop

Workshop	Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Ithaca, USA
Period	20/06/10 → 24/06/10

Cite this

Verbeek, M., Piron, P. G. M., Dullemans, A. M., Cuperus, C., & van der Vlugt, R. A. A. (2010). Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. In Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010 (pp. 39-39). The International Society for Plant Pathology (ISPP).

Verbeek, M. ; Piron, P.G.M. ; Dullemans, A.M. et al. / Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010. The International Society for Plant Pathology (ISPP), 2010. pp. 39-39

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title = "Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y",

abstract = "Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species, which express the transmission efficiency of aphid species in relation to the efficiency of Myzus persicae, the most efficient vector of PVY.In the Netherlands, in the 1980s, aphid{\textquoteright}s REFs were determined using aphids caught alive in the field. Thus, experiments were conducted using limited numbers of aphids and only during the potato growing season. We have now developed a system which allows us to test virus transmission whole year round, using aphid clones reared in insect chambers. Using the new system, we determined the aphids{\textquoteright} relative transmission efficiency factors (REFs) for six isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae showed comparable average transmission efficiencies for all isolates, and was used as an internal control to determine the REFs of 18 other aphid species. The newly determined REFs for PVYN were comparable to previously reported values. New REFs for the PVYNTN strains were overall comparable to the REFs for PVYN, except for Aphis frangulae and Schizaphis graminum. For PVYN-Wi six aphid species showed higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only A. frangulae shows a lower REF for PVYN-Wi. In addition three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determined.",

author = "M. Verbeek and P.G.M. Piron and A.M. Dullemans and C. Cuperus and {van der Vlugt}, R.A.A.",

year = "2010",

language = "English",

pages = "39--39",

booktitle = "Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010",

publisher = "The International Society for Plant Pathology (ISPP)",

note = "Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Ithaca, USA ; Conference date: 20-06-2010 Through 24-06-2010",

}

Verbeek, M, Piron, PGM, Dullemans, AM, Cuperus, C & van der Vlugt, RAA 2010, Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. in Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010. The International Society for Plant Pathology (ISPP), pp. 39-39, Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Ithaca, USA, 20/06/10.

Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. / Verbeek, M.; Piron, P.G.M.; Dullemans, A.M. et al.
Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010. The International Society for Plant Pathology (ISPP), 2010. p. 39-39.

Research output: Chapter in Book/Report/Conference proceeding › Abstract

TY - CHAP

T1 - Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y

AU - Verbeek, M.

AU - Piron, P.G.M.

AU - Dullemans, A.M.

AU - Cuperus, C.

AU - van der Vlugt, R.A.A.

PY - 2010

Y1 - 2010

N2 - Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species, which express the transmission efficiency of aphid species in relation to the efficiency of Myzus persicae, the most efficient vector of PVY.In the Netherlands, in the 1980s, aphid’s REFs were determined using aphids caught alive in the field. Thus, experiments were conducted using limited numbers of aphids and only during the potato growing season. We have now developed a system which allows us to test virus transmission whole year round, using aphid clones reared in insect chambers. Using the new system, we determined the aphids’ relative transmission efficiency factors (REFs) for six isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae showed comparable average transmission efficiencies for all isolates, and was used as an internal control to determine the REFs of 18 other aphid species. The newly determined REFs for PVYN were comparable to previously reported values. New REFs for the PVYNTN strains were overall comparable to the REFs for PVYN, except for Aphis frangulae and Schizaphis graminum. For PVYN-Wi six aphid species showed higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only A. frangulae shows a lower REF for PVYN-Wi. In addition three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determined.

AB - Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species, which express the transmission efficiency of aphid species in relation to the efficiency of Myzus persicae, the most efficient vector of PVY.In the Netherlands, in the 1980s, aphid’s REFs were determined using aphids caught alive in the field. Thus, experiments were conducted using limited numbers of aphids and only during the potato growing season. We have now developed a system which allows us to test virus transmission whole year round, using aphid clones reared in insect chambers. Using the new system, we determined the aphids’ relative transmission efficiency factors (REFs) for six isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae showed comparable average transmission efficiencies for all isolates, and was used as an internal control to determine the REFs of 18 other aphid species. The newly determined REFs for PVYN were comparable to previously reported values. New REFs for the PVYNTN strains were overall comparable to the REFs for PVYN, except for Aphis frangulae and Schizaphis graminum. For PVYN-Wi six aphid species showed higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only A. frangulae shows a lower REF for PVYN-Wi. In addition three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determined.

M3 - Abstract

SP - 39

EP - 39

BT - Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010

PB - The International Society for Plant Pathology (ISPP)

T2 - Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Ithaca, USA

Y2 - 20 June 2010 through 24 June 2010

ER -

Verbeek M, Piron PGM, Dullemans AM, Cuperus C, van der Vlugt RAA. Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. In Plant Viruses: Exploiting Agricultural and Natural Ecosystems. 11th International Plant Virus Epidemiology Symposium and 3rd Workshop of the Plant Virus Ecology Network, Cornell University, Ithaca, USA, 20-24 June 2010. The International Society for Plant Pathology (ISPP). 2010. p. 39-39

Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y

Abstract

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