Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y.

M. Verbeek, P.G.M. Piron, A.M. Dullemans, C. Cuperus, R.A.A. van der Vlugt

    Research output: Contribution to journalArticleAcademicpeer-review

    67 Citations (Scopus)

    Abstract

    Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species. These REFs express the transmission efficiency of aphid species in relation to the transmission efficiency of Myzus persicae, the most efficient vector of PVY. In this paper, we report on the determination of aphids' relative transmission efficiency factors (REFs) for isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae was tested for its transmission efficiency for six PVY isolates (one PVYN, three PVYNTN and two PVYN-Wi isolates) and showed comparable average transmission efficiencies for all isolates. The transmission rate of this biotype for the six PVY isolates was set to 1 and Mp2 was used as an internal control in transmission experiments to determine the REFs of three other biotypes of M. persicae and 16 other aphid species (three biotypes per species when available) for the six PVY isolates. Comparing the calculated REFs for PVYN with the REFs reported in the previous century for PVYN, we observe overall comparable REFs, except for Aphis fabae, Aphis spp., Hyperomyzus lactucae, Macrosiphum euphorbiae and Rhopalosiphum padi, which have a lower REF in our experiments, and Aphis frangulae and Phorodon humuli, which have now a higher REF. Comparing the new REFs found for the PVYNTN strains with the new REFs for PVYN, we observe that they are overall comparable, except for A. frangulae (0.17 compared with 0.53) and Schizaphis graminum (0.05 compared with 0.00). Comparing the REFs calculated for PVYN-Wi with those calculated for PVYN, we can observe six aphid species with higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only the species A. frangulae shows a lower REF for PVYN-Wi compared with the transmission efficiency of PVYN. Three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determine
    Original languageEnglish
    Pages (from-to)39-49
    JournalAnnals of Applied Biology
    Volume156
    Issue number1
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    Potato virus Y
    Aphidoidea
    Aphis (Aphididae)
    biotypes
    Myzus persicae
    Phorodon humuli
    Aphis fabae
    Schizaphis graminum
    Rhopalosiphum padi
    Myzus ascalonicus
    Hyperomyzus lactucae
    Aphis nasturtii
    Aulacorthum solani
    Macrosiphum euphorbiae
    Potyviridae
    Potyvirus
    Acyrthosiphon pisum
    seed tubers
    Solanum tuberosum
    economics

    Keywords

    • helper component protein
    • relative efficiency
    • rhopalosiphum-padi
    • myzus-persicae
    • netherlands
    • potyvirus
    • vectors
    • pvyn
    • behavior
    • virions

    Cite this

    @article{f86f5face71a453ca959eba2f74c65e0,
    title = "Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y.",
    abstract = "Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species. These REFs express the transmission efficiency of aphid species in relation to the transmission efficiency of Myzus persicae, the most efficient vector of PVY. In this paper, we report on the determination of aphids' relative transmission efficiency factors (REFs) for isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae was tested for its transmission efficiency for six PVY isolates (one PVYN, three PVYNTN and two PVYN-Wi isolates) and showed comparable average transmission efficiencies for all isolates. The transmission rate of this biotype for the six PVY isolates was set to 1 and Mp2 was used as an internal control in transmission experiments to determine the REFs of three other biotypes of M. persicae and 16 other aphid species (three biotypes per species when available) for the six PVY isolates. Comparing the calculated REFs for PVYN with the REFs reported in the previous century for PVYN, we observe overall comparable REFs, except for Aphis fabae, Aphis spp., Hyperomyzus lactucae, Macrosiphum euphorbiae and Rhopalosiphum padi, which have a lower REF in our experiments, and Aphis frangulae and Phorodon humuli, which have now a higher REF. Comparing the new REFs found for the PVYNTN strains with the new REFs for PVYN, we observe that they are overall comparable, except for A. frangulae (0.17 compared with 0.53) and Schizaphis graminum (0.05 compared with 0.00). Comparing the REFs calculated for PVYN-Wi with those calculated for PVYN, we can observe six aphid species with higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only the species A. frangulae shows a lower REF for PVYN-Wi compared with the transmission efficiency of PVYN. Three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determine",
    keywords = "helper component protein, relative efficiency, rhopalosiphum-padi, myzus-persicae, netherlands, potyvirus, vectors, pvyn, behavior, virions",
    author = "M. Verbeek and P.G.M. Piron and A.M. Dullemans and C. Cuperus and {van der Vlugt}, R.A.A.",
    year = "2010",
    doi = "10.1111/j.1744-7348.2009.00359.x",
    language = "English",
    volume = "156",
    pages = "39--49",
    journal = "Annals of Applied Biology",
    issn = "0003-4746",
    publisher = "Wiley",
    number = "1",

    }

    Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. / Verbeek, M.; Piron, P.G.M.; Dullemans, A.M.; Cuperus, C.; van der Vlugt, R.A.A.

    In: Annals of Applied Biology, Vol. 156, No. 1, 2010, p. 39-49.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y.

    AU - Verbeek, M.

    AU - Piron, P.G.M.

    AU - Dullemans, A.M.

    AU - Cuperus, C.

    AU - van der Vlugt, R.A.A.

    PY - 2010

    Y1 - 2010

    N2 - Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species. These REFs express the transmission efficiency of aphid species in relation to the transmission efficiency of Myzus persicae, the most efficient vector of PVY. In this paper, we report on the determination of aphids' relative transmission efficiency factors (REFs) for isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae was tested for its transmission efficiency for six PVY isolates (one PVYN, three PVYNTN and two PVYN-Wi isolates) and showed comparable average transmission efficiencies for all isolates. The transmission rate of this biotype for the six PVY isolates was set to 1 and Mp2 was used as an internal control in transmission experiments to determine the REFs of three other biotypes of M. persicae and 16 other aphid species (three biotypes per species when available) for the six PVY isolates. Comparing the calculated REFs for PVYN with the REFs reported in the previous century for PVYN, we observe overall comparable REFs, except for Aphis fabae, Aphis spp., Hyperomyzus lactucae, Macrosiphum euphorbiae and Rhopalosiphum padi, which have a lower REF in our experiments, and Aphis frangulae and Phorodon humuli, which have now a higher REF. Comparing the new REFs found for the PVYNTN strains with the new REFs for PVYN, we observe that they are overall comparable, except for A. frangulae (0.17 compared with 0.53) and Schizaphis graminum (0.05 compared with 0.00). Comparing the REFs calculated for PVYN-Wi with those calculated for PVYN, we can observe six aphid species with higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only the species A. frangulae shows a lower REF for PVYN-Wi compared with the transmission efficiency of PVYN. Three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determine

    AB - Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species. These REFs express the transmission efficiency of aphid species in relation to the transmission efficiency of Myzus persicae, the most efficient vector of PVY. In this paper, we report on the determination of aphids' relative transmission efficiency factors (REFs) for isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae was tested for its transmission efficiency for six PVY isolates (one PVYN, three PVYNTN and two PVYN-Wi isolates) and showed comparable average transmission efficiencies for all isolates. The transmission rate of this biotype for the six PVY isolates was set to 1 and Mp2 was used as an internal control in transmission experiments to determine the REFs of three other biotypes of M. persicae and 16 other aphid species (three biotypes per species when available) for the six PVY isolates. Comparing the calculated REFs for PVYN with the REFs reported in the previous century for PVYN, we observe overall comparable REFs, except for Aphis fabae, Aphis spp., Hyperomyzus lactucae, Macrosiphum euphorbiae and Rhopalosiphum padi, which have a lower REF in our experiments, and Aphis frangulae and Phorodon humuli, which have now a higher REF. Comparing the new REFs found for the PVYNTN strains with the new REFs for PVYN, we observe that they are overall comparable, except for A. frangulae (0.17 compared with 0.53) and Schizaphis graminum (0.05 compared with 0.00). Comparing the REFs calculated for PVYN-Wi with those calculated for PVYN, we can observe six aphid species with higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only the species A. frangulae shows a lower REF for PVYN-Wi compared with the transmission efficiency of PVYN. Three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determine

    KW - helper component protein

    KW - relative efficiency

    KW - rhopalosiphum-padi

    KW - myzus-persicae

    KW - netherlands

    KW - potyvirus

    KW - vectors

    KW - pvyn

    KW - behavior

    KW - virions

    U2 - 10.1111/j.1744-7348.2009.00359.x

    DO - 10.1111/j.1744-7348.2009.00359.x

    M3 - Article

    VL - 156

    SP - 39

    EP - 49

    JO - Annals of Applied Biology

    JF - Annals of Applied Biology

    SN - 0003-4746

    IS - 1

    ER -