Detection of varicella-zoster virus and herpes simplex virus by the polymerase chain reaction with degenerate primers

J.J.L. Jacobs, E. Folkers, J. Vreeswijk

    Research output: Contribution to journalArticleAcademicpeer-review

    3 Citations (Scopus)

    Abstract

    Varicella-zoster virus (VZV) and herpes simplex virus (HSV) are human pathogens of significance involved in multiple diseases with either typical or atypical clinical features. In neonates and immunocompromised patients these alphaherpesviruses may cause life-threatening diseases such as encephalitis. Detection of VZV by virus culture is difficult. Polymerase chain reaction (PCR) is quicker and more sensitive and applicable in most clinical microbiological laboratories. Using degenerate primers, glycoprotein B (gB) DNA was amplified from all alphaherpesvirus field strains present in clinical samples. The amplification of gB allowed virus typing of VZV, HSV-1 and HSV-2 using restriction enzyme digestion of the PCR products. Degenerate primers can replace conventional primers in diagnostic PCR without loss of sensitivity and specificity.
    Original languageEnglish
    Pages (from-to)155-167
    JournalJournal of Virological Methods
    Volume83
    Issue number1-2
    DOIs
    Publication statusPublished - 1999

    Fingerprint

    Dive into the research topics of 'Detection of varicella-zoster virus and herpes simplex virus by the polymerase chain reaction with degenerate primers'. Together they form a unique fingerprint.

    Cite this