Detection of plasmid transfer from Pseudomonas fluorescens to indigenous bacteria in soil by using Bacteriophage 0R2f for donor counterselection.

E. Smit, J.D. van Elsas, J.A. van Veen, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

70 Citations (Scopus)

Abstract

The transfer of a genetically marked derivative of plasmid RP4, RP4p, from Pseudomonas fluorescens to members of the indigenous microflora of the wheat rhizosphere was studied by using a bacteriophage that specifically lyses the donor strain and a specific eukaryotic marker on the plasmid. Transfer of RP4p to the wheat rhizosphere microflora was observed, and the number of transconjugants detected was approximately 103 transconjugants per g of soil when 107 donor cells per g of soil were added; transfer in the corresponding bulk soil was slightly above the limit of detection. All of the indigenous transconjugants which we analyzed contained a 60-kb plasmid and were able to transfer this plasmid to a Nxr RprP. fluorescens recipient strain. The indigenous transconjugants were identified as belonging to Pseudomonas spp., Enterobacter spp., Comamonas spp., and Alcaligenes spp.
Original languageEnglish
Pages (from-to)3482-3488
JournalApplied and Environmental Microbiology
Volume57
Issue number12
Publication statusPublished - 1991

Fingerprint Dive into the research topics of 'Detection of plasmid transfer from Pseudomonas fluorescens to indigenous bacteria in soil by using Bacteriophage 0R2f for donor counterselection.'. Together they form a unique fingerprint.

Cite this